Back to results
US 6,602,991 B1Granted
Process for preparing a charcoal-GM1 complex
AssigneeTRB Pharma Industria Quimica e Farmaceutica Ltda
InventorsSilvia Storpirtis, Pedro Gonçalves De Oliveira
Life of this patent
Estimating the term…
Abstract
The invention provides a process to prepare a complex of adsorption charcoal-GM1 stable at different pH values. This process is based on the suspension method, the formulation example being presented. An analytical method to determine non derivatized GM1 in aqueous solutions using the high efficiency liquid chromatography technique is also object of the present invention.
Claims2
Process for preparing a charcoal-GM1 complex, characterized by comprising the stages of:
- (A) preparing a diluente solution (A) composed of 0 to 0.900% of sodium chloride, from 0 to 0.030% of monobasic sodium phosphate-2H2O, and 0 to 0.400% of dibasic sodium phosphate-12H2O, dissolved in distilled water;
- (b) dissolving, in part of the solution (A) from 0.200 to 4.000% of GM1, then obtaining a new solution (B);
- (c) suspending activated charcoal in the other part of the solution (A) in the proportion of 1:3 to 1:7 w/v, starting and maintaining moderated agitation for a period of 5 to 15 minutes;
- (d) slowly adding under agitation, adequate quantities of the solution (B) in such way as to obtain proportions varying from 1:100 and 1:10 (GM1:charcoal), and the agitation is maintained by a period of 10 to 20 minutes;
- (e) filtering the suspension, washing the complex with the solution (A) and submitting it to drying;
- and,
- (f) passing the dry complex through a 40 “mesh” sieve and storing the product in a hermetically sealed recipient.
4 more elements
Dependencies are inferred from the claim text.