Enterotoxin-deficient bacillus
AssigneeWisconsin Alumni Research Foundation
InventorsJo Handelsman, Amy K. Klimowicz
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Abstract
A enterotoxin-deficient mutant of a member strain of the Bacillus cereus group does not produce HBL enterotoxin, which has been regarded as a human pathogen found in member strains. An enterotoxin-deficient mutant is suitable for use as a biocontrol agent. Methods for making the mutant and for using the mutant are described.
Claims14
A method for obtaining a mutant Bacillus, the method comprising the steps of:
- mutating a Bacillus to introduce a mutation in hblA that prevents formation of an active Niemolysin BL enterotoxin;
- and
- selecting a mutant Bacillus comprising the mutation,
- wherein the Bacillus is selected from the group consisting of B. cereus, B. thuringiensis and B. anthracis.
A method for obtaining a mutant Bacillus cereus AS4-12,the method comprising the steps of:
- mutating strain AS4-12 to introduce a mutation in hblA that prevents formation of an active Hemolysin BL enterotoxin;
- and
- selecting a mutant of strain AS-12 that comprises the mutation.
A Bacillus having a mutation in hblA that prevents formation of an active Hemolysin BL enterotoxin, wherein the Bacillus supresses damping-off of tomato seedlings caused by Pythium torulosum in a growth chamber assay and is selected from the group consisting of B. cereus, B. thuringiensis and B. anthracis.
Dependencies are inferred from the claim text.