Back to results
US 6,602,711 B1Granted
Method of making embryoid bodies from primate embryonic stem cells
AssigneeWisconsin Alumni Research Foundation
InventorsJames A Thomson, Vivienne S. Marshall, Jennifer J. Swiergiel
Life of this patent
Estimating the term…
Abstract
Primate embryoid bodies are formed from primate ES cells. The ES cells form clumps. One then removes the clumps, as clumps, and permits incubation under non-adherent conditions. The development of embryoid bodies from primate ES cells is dependent on maintaining the aggregation of cells, as individualized cells will rapidly die.
Claims10
A method for producing primate embryoid bodies from colonies of primate embryonic stem cells that are adhering to a substrate, the method comprising:
- removing the adhering colonies of the embryonic stem cells from the substrate in clumps;
- and
- then incubating the clumps in a container under conditions in which the clumps are essentially inhibited from attaching to the container and under conditions in which the clumps of embryonic stem cells coalesce into embryoid bodies.
Dependencies are inferred from the claim text.