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US 6,602,707 B2Granted
Mammalian cell surface DNA receptor-encoding nucleic acid
AssigneeOregon Health & Science University
InventorsSteven Hefeneider, Robert M. Bennett
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AssigneeOregon Health & Science University
InventorsSteven Hefeneider, Robert M. Bennett
Estimating the term…
The present invention relates to novel mammalian DNA-R proteins and genes that encode such proteins. The invention is directed toward the isolation and characterization of mammalian DNA-R proteins. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to rat and human homologues of a mammalian DNA-R gene. Also provided are recombinant expression constructs capable of expressing the mammalian DNA-R genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the mammalian catecholamine receptor proteins encoded therein. The invention also provides methods for screening compounds in vitro that are capable of binding to the mammalian DNA-R proteins of the invention, and further characterizing the binding properties of such compounds in comparison with known DNA-R agonists and antagonists. Improved methods of pharmacological screening are provided thereby.
An isolated nucleic acid comprising a nucleotide sequence encoding a mammalian cell surface DNA receptor (DNA-R) having an amino acid sequence identified by SEQ ID No. 2.
An isolated nucleic acid encoding a DNA-binding fragment of a mammalian DNA-R having an amino acid sequence identified by SEQ ID No. 2.
An isolated nucleic acid encoding a soluble mammalian DNA-R, wherein the mammalian DNA-R is a human DNA-R having an amino acid sequence identified by SEQ ID No.:2 wherein amino acids 1133-1171 arc deleted therefrom.
Dependencies are inferred from the claim text.