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AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsXin-Liang Li, Lars G. Ljungdahl, Michael J. Azain, Edward T. Davies, Ashit K. Shah, David L. Blum, Irina Kataeva
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Described herein are four phenolic acid esterases, three of which correspond to domains of previously unknown function within bacterial xylanases, from XynY and XynZ of Clostridium thermocellum and from a feruloyl esterase of Ruminococcus. The fourth specifically exemplified phenolic acid esterase is a protein encoded within the genome of Orpinomyces PC-2. The amino acids of these polypeptides and nucleotide sequences encoding them are provided. Recombinant host cells, expression vectors and methods for the recombinant production of phenolic acid esterases are also provided. Further provided are methods for improving nutrient availability and ferulic acid availability when food or feed, or other material is treated with a phenolic acid esterase, desirably in combination with a xylanase.
A method for increasing free ferulic acid content of a plant-derived composition, said method comprising the step of contacting a plant-derived composition with a phenolic acid esterase wherein said phenolic acid esterase comprises the amino acid sequence of SEQ ID NO:18, amino acids 1 to 530 or wherein the phenolic acid esterase consists of an amino acid sequence selected from the group consisting of amino acids 795 to 1077 of SEQ ID NO:12, amino acids 546 to 789 of SEQ ID NO:16, amino acids 20 to 286 of SEQ ID NO:14, amino acids 20 to 307 of SEQ ID NO:14, and amino acids 20 to 421 of SEQ ID NO:14.
Dependencies are inferred from the claim text.