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US 6,602,422 B1Granted
Micro column system
AssigneeMiltenyi Biotech GmbH
InventorsStefan Miltenyi, Gregor Siebenkotten, Mathias Koester
Life of this patent
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Abstract
A separation and release process for purifying biological material on a micro separation column includes release of the biological material from magnetic carriers and elution from the micro separation column while the magnetic carriers are still magnetically retained by a matrix of ferromagnetic particles inside the micro separation column.
Claims14
A process for purifying biological material on a column, comprising:
- retaining magnetic carriers bound to the biological material with ferromagnetic particles in a magnetic field in first and second tubular portions of a micro separation column, wherein said first and second tubular portions have different cross-sectional areas, each cross-sectional area having a substantially constant diameter;
- and
- eluting the biological material by dissociating the biological material from the magnetic carriers while still in a magnetic field.
A process for purifying biological material on a column, comprising:
- providing a separation column containing a matrix including ferromagnetic material therein wherein said separation column includes first and second tubular portions, said first and second tubular portions have different cross-sectional areas, each cross-sectional area having a substantially constant diameter;
- generating a high gradient magnetic field in the matrix;
- flowing a fluid containing magnetic carriers bound to the biological material through the separation column, whereby the magnetic carriers are retained by the ferromagnetic material;
- and
- eluting the biological material by dissociating the biological material from the magnetic carriers while still in said magnetic field.
2 more elements
A process for purifying biological material on a column, said process comprising the steps of:
- retaining superparamagnetic carriers bound to the biological material with ferromagnetic particles in a magnetic field in a micro-separation column having first and second tubular portions, wherein said first and second tubular portions have different cross-sectional areas, each cross-sectional area having a substantially constant diameter;
- and
- eluting the biological material by dissociating the biological material from the superparamagnetic carriers while still in said magnetic field.
Dependencies are inferred from the claim text.