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US 6,602,400 B1Granted
Method for enhanced bio-conjugation events
AssigneeMotorola, Inc.
InventorsVi-En Choong, Changming Li, Song Shi, George Maracas
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Abstract
The present invention provides an apparatus and methods for efficient, high-throughput electrical or electrochemical detection of biomolecules. More specifically, the invention provides an apparatus in which an independent set of electrodes is used to increase the occurrence of a desired bio-conjugation event at a test site.
Claims20
A method for the electrical or electrochemical detection of molecular interactions between an immobilized probe molecule and a target molecule, comprising:
- (a) providing a substrate comprising at least one test site electrode comprising immobilized probe molecules;
- (b) contacting said test site electrode with a sample mixture containing a particular target molecule;
- (c) concentrating said target molecules by applying an electrical potential between a pair of manipulation electrodes such that bioconjugation between said immobilized probe molecules and said target molecule is enhanced, and wherein said bioconjugation forms a conjugated complex, wherein said conjugated complex does not comprise a reporter group;
- (d) detecting an electrical signal at said test site electrode;
- and
- (e) determining whether said target molecule is present.
3 more elements
Dependencies are inferred from the claim text.