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US 6,599,729 B2Granted
α-agarase and process for producing the same
AssigneeTakara Shuzo Co., Ltd.
InventorsJun Tomono, Yoshiko Nomura, Hiroaki Sagawa, Takeshi Sakai, Ikunoshin Kato
Life of this patent
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Abstract
A polypeptide having an α-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.
Claims7
An isolated gene encoding a polypeptide having an α-agarase activity, selected from the group consisting of:
- (1) a gene encoding a polypeptide containing an amino acid sequence consisting of 586 residues from residue 365 to residue 950 of SEQ ID NO:15;
- (2) a gene containing a nucleotide sequence consisting of 1758 nucleotides from nucleotide 1093 to nucleotide 2850 of SEQ ID NO:13;
- and
- (3) a gene that hybridizes to said gene of (2) in a solution containing 6× SSC, 0.5% SDS, 5× Denhart's and 100 mg/ml herring sperm DNA at 65° C.
1 more element
Dependencies are inferred from the claim text.