Nucleotide and protein sequence of mammastatin and methods of use
AssigneeThe Regents of the University of Michigan
InventorPaul R. Ervin, Jr.
Life of this patent
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Abstract
A nucleic acid sequence encoding Mammastatin, a specific mammary cell growth inhibitor. Mammastatin is encoded by a single nucleic acid sequence and has an approximate molecular weight of 44 kDa in its inactive, non-phosphorylated form. Normal mammary cells express functional phosphorylated forms having approximate molecular weights of 53 kDa and 49 kDa. Metastatic mammary cells either do not express Mammastatin at all, or do not express the 53 kDa or 49 kDa, phosphorylated forms. Mammary cancer cells are inhibited in their growth by the administration of phosphorylated mammastatin.
Claims9
A method for the diagnosis of breast cancer, comprising:
- quantifying an amount of Mammastatin in a patient's sample of mammary cells, mammary tissue, or fluid, wherein Mammastatin comprises a mammary cell growth inhibitory protein encoded by SEQ. ID. NO:
- 1;
- and
- correlating an amount of Mammastatin in the patient's sample that is less than a control amount of Mammastatin from a normal, non-breast cancer female with the presence of or predispositon to breast cancer.
The method according to clam 4, wherein the immunoassay employs monoclonal antibody 7G6.
A method for the diagnosis of breast cancer, comprising;
- quantifying an amount of Mammastatin in a patient's blood, wherein Mammastatin comprises a mammary cell growth inhibitory protein encoded by SEQ. ID. NO:
- 1;
- and
- correlating an amount of Mammastatin in the patient's blood that is less than about 10 ng/ml with presence of or predisposition to breast cancer.
A method for the detection of breast cancer, comprising:
- quantifying an amount of Mammastatin in a first sample of a patient's mammary cells, mammary tissue, or fluid, and in a second sample from the same patient obtained later in time, wherein Mammastatin comprises a mammary cell growth inhibitory protein encoded by SEQ. ID. NO:
- 1;
- and
- correlating an increase or decrease of Mammastatin in the second sample relative to the first sample with an increase or decrease in breast cancer.
Dependencies are inferred from the claim text.