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US 6,599,441 B1Granted
Crystallization solutions
AssigneeEmerald BioStructures, Inc.
InventorsMary Ann Chrislip, Bart Staker
Life of this patent
Estimating the term…
Abstract
In one aspect, the present invention provides sets of crystallization solutions that each comprise a crystallization solution set selected from the group of crystallization solution sets consisting of Crystallization Solution Set 1, Crystallization Solution Set 2, Crystallization Solution Set 3, Crystallization Solution Set 4 and Crystallization Solution Set 5. In another aspect, the present invention provides kits that each comprise at least one crystallization plate and a Crystallization Solution Set of the invention.
Claims17
A set of crystallization solutions comprising a crystallization solution set selected from the group of crystallization solution sets consisting of:
- Solution Compound Buffer Precipitant Salt (a) Crystallization Solution Set 1:
- 1 100.000 mM HEPES/NaOH pH:
- 10.000% v/v 200.000 mM 7.5 isopropanol sodium chloride 2 100.000 mM Na3 citrate/citric acid 20.000% w/v pH:
- 5.5 PEG-3000 3 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-8000 ZnAc2 4 100.000 mM Na3 citrate/citric acid 2000.000 pH:
- 5.5 mM (NH4)2 sulfate 5 100.000 mM Tris base/HCl pH:
- 7. 20.000% w/v PEG-2000 MME 6 100.000 mM MES/NaOH pH:
- 6. 20.000% v/v 200.000 mM 1,4-butane- Li2 sulfate diol 7 100.000 mM imidazole/HCl pH:
- 8. 20.000% w/v 200.000 mM PEG-1000 CaAc2 8 100.000 mM Na2 H phosphate/K 2500.000 H2 phosphate pH:
- 6.2 mM sodium chloride 9 100.000 mM acetic acid/NaAc pH:
- 30.000% w/v 200.000 mM 4.5 PEG-8000 Li2 sulfate 10 100.000 mM Tris base/HCl pH:
- 10.000% v/v 8.5 isopropanol 11 100.000 mM Tris base/HCl pH:
- 30.000% v/v 200.000 mM 8.5 PEG-400 MgCl2 12 100.000 mM imidazole/HCl pH:
- 8. 2500.000 mM sodium chloride 13 100.000 mM MES/NaOH pH:
- 6. 10.000% v/v 200.000 mM isopropanol CaAc2 14 100.000 mM Tris base/HCl pH:
- 7. 15.000% v/v EtOH 15 100.000 mM acetic acid/NaAc pH:
- 30.000% v/v 200.000 mM 4.5 PEG-400 CaAc2 16 100.000 mM acetic acid/NaAc pH:
- 20.000% w/v 200.000 mM 4.5 PEG-1000 ZnAc2 17 100.000 mM acetic acid/NaAc pH:
- 10.000% w/v 200.000 mM 4.5 PEG-3000 ZnAc2 18 100.000 mM Na2 H phosphate/K 10.000% w/v 200.000 mM H2 phosphate pH:
- 6.2 PEG-8000 sodium chloride 19 100.000 mM Tris base/HCl pH:
- 1000.000 8.5 mM (NH4)2 H phosphate 20 100.000 mM Na cacodylate/HCl 10.000% v/v 200.000 mM pH:
- 6.5 isopropanol ZnAc2 21 100.000 mM Na cacodylate/HCl 30.000% v/v 200.000 mM pH:
- 6.5 PEG-400 Li2 sulfate 22 100.000 mM Na3 citrate/citric acid 15.000% v/v 200.000 mM pH:
- 5.5 EtOH Li2 sulfate 23 100.000 mM HEPES/NaOH pH:
- 1260.000 7.5 mM (NH4)2 sulfate 24 100.000 mM Tris base/HCl pH:
- 7. 2500.000 200.000 mM mM sodium MgCl2 chloride 25 100.000 mM MES/NaOH pH:
- 6. 15.000% v/v 200.000 mM EtOH ZnAc2 26 100.000 mM imidazole/HCl pH:
- 8. 10.000% v/v isopropanol 27 100.000 mM HEPES/NaOH pH:
- 15.000% v/v 200.000 mM 7.5 EtOH MgCl2 28 100.000 mM Na cacodylate/HCl 10.000% w/v 200.000 mM pH:
- 6.5 PEG-3000 MgCl2 29 100.000 mM MES/NaOH pH:
- 6. 20.000% w/v 200.000 mM PEG-8000 CaAc2 30 100.000 mM CHES/NaOH pH:
- 1260.000 200.000 mM 9.5 mM (NH4)2 sodium sulfate chloride 31 100.000 mM imidazole/HCl pH:
- 8. 20.000% v/v 200.000 mM 1,4-butane- ZnAc2 diol 32 100.000 mM Tris base/HCl pH:
- 7. 1000.000 200.000 mM mM Na3 sodium citrate chloride 33 100.000 mM HEPES/NaOH pH:
- 30.000% v/v 200.000 mM 7.5 PEG-400 sodium chloride 34 100.000 mM Tris base/HCl pH:
- 7. 10.000% w/v 200.000 mM PEG-8000 MgCl2 35 100.000 mM Na cacodylate/HCl 20.000% w/v 200.000 mM pH:
- 6.5 PEG-1000 MgCl2 36 100.000 mM imidazole/HCl pH:
- 8. 2500.000 200.000 mM mM sodium ZnAc2 chloride 37 100.000 mM Na2 H phosphate/K 40.000% v/v H2 phosphate pH:
- 6.2 EG 38 100.000 mM Tris base/HCl pH:
- 30.000% v/v 200.000 mM 8.5 PEG-200 (NH4)2 H phosphate 39 100.000 mM HEPES/NaOH pH:
- 10.000% v/v 50.000 mM 7.5 glycerol Li2 sulfate 30.000% v/v PEG-600 40 100.000 mM HEPES/NaOH pH:
- 40.000% v/v 7.5 EG 5.000% w/v PEG-3000 41 100.000 mM Tris base/HCl pH:
- 7. 40.000% v/v EG 42 100.000 mM Tris base/HCl pH:
- 50.000% v/v 200.000 mM 8.5 EG MgCl2 43 100.000 mM Tris base/HCl pH:
- 5.000% w/v 8.5 PEG-8000 10.000% v/v glycerol 20.000% v/v PEG-300 44 100.000 mM acetic acid/NaAc pH:
- 40.000% v/v 4.5 PEG-400 45 100.000 mM Tris base/HCl pH:
- 7. 10.000% v/v 500.000 mM glycerol (NH4)2 30.000% v/v sulfate PEG-600 46 100.000 mM HEPES/NaOH pH:
- 50.000% v/v 200.000 mM 7.5 EG Li2 sulfate 47 100.000 mM acetic acid/NaAc pH:
- 30.000% v/v 100.000 mM 4.5 PEG-200 sodium chloride 48 100.000 mM CAPS/NaOH pH:
- 30.000% v/v 200.000 mM 10.5 PEG-200 (NH4)2 sulfate (b) Crystallization Solution Set 2:
- 1 100.000 mM Tris base/HCl pH:
- 7. 10.000% v/v 200.000 mM isopropanol ZnAc2 2 100.000 mM acetic acid/NaAc pH:
- 10.000% v/v 200.000 mM 4.5 isopropanol CaAc2 3 100.000 mM Na3 citrate/Na3 10.000% v/v 200.000 mM citrate pH:
- 5.5 isopropanol Li2 sulfate 4 100.000 mM Tris base/HCl pH:
- 7. 10.000% v/v 200.000 mM isopropanol CaAc2 5 100.000 mM acetic acid/NaAc pH:
- 10.000% v/v 200.000 mM 4.5 isopropanol sodium chloride 6 100.000 mM Na3 citrate/Na3 10.000% v/v 200.000 mM citrate pH:
- 5.5 isopropanol MgCl2 7 100.000 mM HEPES/NaOH pH:
- 10.000% w/v 200.000 mM 7.5 PEG-8000 sodium chloride 8 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-8000 ZnAc2 9 100.000 mM Na2 H phosphate/K 10.000% w/v 200.000 mM H2 phosphate pH:
- 6.2 PEG-8000 Li2 sulfate 10 100.000 mM acetic acid/NaAc pH:
- 10.000% w/v 200.000 mM 4.5 PEG-8000 CaAc2 11 100.000 mM Tris base/HCl pH:
- 10.000% w/v 200.000 mM 8.5 PEG-8000 MgCl2 12 100.000 mM Na cacodylate/HCl 10.000% w/v 200.000 mM pH:
- 6.5 PEG-8000 sodium chloride 13 100.000 mM Na3 citrate/citric acid 10.000% w/v 200.000 mM pH:
- 5.5 PEG-8000 Li2 sulfate 14 100.000 mM HEPES/NaOH pH:
- 10.000% w/v 200.000 mM 7.5 PEG-8000 MgCl2 15 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 200.000 mM 7.5 PEG-2000 NaCl MME 16 100.000 mM MES/NaOH pH:
- 6. 20.000% w/v 200.000 mM PEG-2000 ZnAc2 MME 17 100.000 mM Na2 H phosphate/K 20.000% w/v H2 phosphate pH:
- 6.2 PEG-2000 MME 200.000 mM Li2 sulfate 18 100.000 mM acetic acid/NaAc pH:
- 20.000% w/v 200.000 mM 4.5 PEG-2000 CaAc2 MME 19 100.000 mM Tris base/HCl pH:
- 20.000% w/v 200.000 mM 8.5 PEG-2000 MgCl2 MME 20 100.000 mM cacodylic acid/Na 20.000% w/v cacodylate pH:
- 6.5 PEG-2000 MME 200.000 mM Li2 sulfate 21 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 7.5 PEG-2000 MME 200.000 mM Li2 sulfate 22 100.000 mM Tris base/HCl pH:
- 20.000% v/v 8.5 1,4-butane- diol 200.000 mM Li2 sul- fate 23 100.000 mM cacodylic acid/Na 20.000% v/v 200.000 mM cacodylate pH:
- 6.5 1,4-butane- CaAc2 diol 24 100.000 mM Na3 citrate/citric acid 20.000% v/v 200.000 mM pH:
- 5.5 1,4-butane- MgCl2 diol (c) Crystallization Solution Set 3:
- 1 100.000 mM Tris base/HCl pH:
- 7. 20.000% v/v 200.000 mM 1,4-butane- ZnAc2 diol 2 100.000 mM Tris base/HCl pH:
- 20.000% v/v 200.000 mM 8.5 1,4-butane- MgCl2 diol 3 100.000 mM Na3 citrate/citric acid 20.000% v/v 200.000 mM pH:
- 5.5 1,4-butane- sodium diol chloride 4 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 200.000 mM 7.5 PEG-1000 sodium chloride 5 100.000 mM MES/NaOH pH:
- 6 20.000% w/v 200.000 mM PEG-1000 ZnAc2 6 100.000 mM Tris base/HCl pH:
- 7. 20.000% w/v 200.000 mM PEG-1000 Li2 sulfate 7 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 200.000 mM 7.5 PEG-1000 MgCl2 8 100.000 mM MES/NaOH pH:
- 6. 20.000% w/v 200.000 mM PEG-1000 sodium chloride 9 100.000 mM HEPES/NaOH pH:
- 2500.000 7.5 mM sodium chloride 10 100.000 mM MES/NaOH pH:
- 6. 2500.000 200.000 mM mM sodium ZnAc2 chloride 11 100.000 mM Tris base/HCl pH:
- 2500.000 200.000 mM 8.5 mM sodium Li2 sulfate chloride 12 100.000 mM cacodylic acid/Na 2500.000 200.000 mM cacodylate pH:
- 6.5 mM sodium CaAc2 chloride 13 100.000 mM Na3 citrate/citric acid 2500.000 200.000 mM pH:
- 5.5 mM sodium MgCl2 chloride 14 100.000 mM cacodylic acid/Na 2500.000 200.000 mM cacodylate pH:
- 6.5 mM sodium Li2 sulfate chloride 15 100.000 mM Tris base/HCl pH:
- 7. 30.000% v/v 200.000 mM PEG-400 ZnAc2 16 100.000 mM imidazole/HCl pH:
- 8. 30.000% v/v 200.000 mM PEG-400 Li2 sulfate 17 100.000 mM Na3 citrate/citric acid 30.000% v/v 200.000 mM pH:
- 5.5 PEG-400 MgCl2 18 100.000 mM Na3 citrate/citric acid 30.000% v/v 200.000 mM pH:
- 5.5 PEG-400 Li2 sulfate 19 100.000 mM imidazole/HCl pH:
- 8. 30.000% v/v 200.000 mM PEG-400 ZnAc2 20 100.000 mM Tris base/HCl pH:
- 7. 30.000% v/v 200.000 mM PEG-400 NaCl 21 100.000 mM Na2 H phosphate/K 15.000% v/v 200.000 mM H2 phosphate pH:
- 6.2 EtOH sodium chloride 22 100.000 mM acetic acid/NaAc pH:
- 15.000% v/v 200.000 mM 4.5 EtOH ZnAc2 23 100.000 mM cacodylic acid/Na 15.000% v/v 200.000 mM cacodylate pH:
- 6.5 EtOH CaAc2 24 100.000 mM acetic acid/NaAc pH:
- 15.000% v/v 200.000 mM 4.5 EtOH MgCl2 (d) Crystallization Solution Set 4:
- 1 100.000 mM cacodylic acid/Na 15.000% v/v 200.000 mM cacodylate pH:
- 6.5 EtOH sodium chloride 2 100.000 mM HEPES/HEPES pH:
- 10.000% w/v 200.000 mM 7.5 PEG-3000 sodium chloride 3 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-3000 ZnAc2 4 100.000 mM Tris base/HCl pH:
- 10.000% w/v 200.000 mM 8.5 PEG-3000 CaAc2 5 100.000 mM Na3 citrate/citric acid 10.000% w/v 200.000 mM pH:
- 5.5 PEG-3000 MgCl2 6 100.000 mM Tris base/HCl pH:
- 10.000% w/v 200.000 mM 8.5 PEG-3000 Li2 sulfate 7 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-3000 Li2 sulfate 8 100.000 mM HEPES/HEPES pH:
- 1000.000 200.000 mM 7.5 mM (NH4)2 sodium H phosphate chloride 9 100.000 mM cacodylic acid/Na 1000.000 200.000 mM cacodylate pH:
- 6.5 mM (NH4)2 sodium H phosphate chloride 10 100.000 mM Na2 H phosphate/K 1260.000 200.000 mM H2 phosphate pH:
- 6.2 mM (NH4)2 Li2 sulfate sulfate 11 100.000 mM Tris base/HCl pH:
- 7. 1260.000 200.000 mM mM (NH4)2 MgCl2 sulfate 12 100.000 mM imidazole/HCl pH:
- 8. 1260.000 200.000 mM mM (NH4)2 Li2 sulfate sulfate 13 100.000 mM Na3 citrate/citric acid 1260.000 200.000 mM pH:
- 5.5 mM (NH4)2 sodium sulfate chloride 14 100.000 mM HEPES/HEPES pH:
- 20.000% w/v 200.000 mM 7.5 PEG-8000 sodium chloride 15 100.000 mM Tris base/HCl pH:
- 7. 20.000% w/v 200.000 mM PEG-8000 ZnAc2 16 100.000 mM imidazole/HCl pH:
- 8. 20.000% w/v 200.000 mM PEG-8000 Li2 sulfate 17 100.000 mM acetic acid/NaAc pH:
- 20.000% w/v 200.000 mM 4.5 PEG-8000 MgCl2 18 100.000 mM cacodylic acid/Na 20.000% w/v 200.000 mM cacodylate pH:
- 6.5 PEG-8000 sodium chloride 19 100.000 mM Tris base/HCl pH:
- 7. 20.000% w/v 200.000 mM PEG-8000 MgCl2 20 100.000 mM HEPES/HEPES pH:
- 1000.000 200.000 mM 7.5 mM Na3 sodium citrate chloride 21 100.000 mM MES/NaOH pH:
- 6. 1000.000 200.000 mM mM Na3 ZnAc2 citrate 22 100.000 mM imidazole/HCl pH:
- 8. 1000.000 200.000 mM mM Na3 Li2 sulfate citrate 23 100.000 mM acetic acid/NaAc pH:
- 1000.000 200.000 mM 4.5 mM Na3 MgCl2 citrate 24 100.000 mM Tris base/HCl pH:
- 1000.000 200.000 mM 8.5 mM Na3 Li2 sulfate citrate (e) Crystallization Solution Set 5:
- 1 100.000 mM MES/NaOH pH:
- 6. 1000.000 200.000 mM mM Na3 MgCl2 citrate 2 100.000 mM acetic acid/NaAc pH:
- 10.000% v/v 200.000 mM 4.5 isopropanol Li2 sulfate 3 100.000 mM Na3 citrate/citric acid 10.000% v/v 200.000 mM pH:
- 5.5 isopropanol sodium chloride 4 100.000 mM acetic acid/NaAc pH:
- 10.000% w/v 200.000 mM 4.5 PEG-8000 MgCl2 5 100.000 mM Tris base/HCl pH:
- 10.000% w/v 200.000 mM 8.5 PEG-8000 sodium chloride 6 100.000 mM acetic acid/NaAc pH:
- 20.000% w/v 200.000 mM 4.5 PEG-2000 ZnAc2 MME 7 100.000 mM Tris base/HCl pH:
- 20.000% w/v 200.000 mM 8.5 PEG-2000 Li2 sulfate MME 8 100.000 mM Tris base/HCl pH:
- 20.000% w/v 200.000 mM 8.5 1,4-butane- sodium diol chloride 9 100.000 mM cacodylic acid/Na 20.000% w/v 200.000 mM cacodylate pH:
- 6.5 1,4-butane- Li2 sulfate diol 10 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 200.000 mM 7.5 PEG-1000 Li2 sulfate 11 100.000 mM MES/NaOH pH:
- 6. 20.000% w/v 200.000 mM PEG-1000 MgCl2 12 100.000 mM MES/NaOH pH:
- 6. 2500.000 200.000 mM nM sodium Li2 sulfate chloride 13 100.000 mM Tris base/HCl pH:
- 30.000% w/v 200.000 mM 8.5 PEG-8000 Li2 sulfate 14 100.000 mM cacodylic acid/Na 30.000% w/v 200.000 mM cacodylate pH:
- 6.5 PEG-8000 CaAc2 15 100.000 mM MES/NaOH pH:
- 6. 30.000% w/v 200.000 mM PEG-400 MgCl2 16 100.000 mM Tris base/HCl pH:
- 7. 30.000% w/v 200.000 mM PEG-400 Li2 sulfate 17 100.000 mM HEPES/NaOH pH:
- 10.000% w/v 200.000 mM 7.5 PEG-3000 Li2 sulfate 18 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-3000 MgCl2 19 100.000 mM imidazole/HCl pH:
- 8. 1000.000 200.000 mM mM Na3 MgCl2 citrate 20 100.000 mM Tris base/HCl pH:
- 1000.000 200.000 mM 8.5 mM Na3 sodium citrate chloride 21 100.000 mM Tris base/HCl pH:
- 7. 2500.000 mM NaMal 22 100.000 mM acetic acid/NaAc pH:
- 2500.000 4.5 mM NaMal 23 100.000 mM Tris base/HCl pH:
- 2500.000 8.5 mM NaMal 24 100.000 mM imidazole/HCl pH:
- 8. 2500.000 mM NaMal.
147 more elements
A kit comprising at least one crystallization plate and a set of crystallization solutions comprising a set of crystallization solutions selected from the group of crystallization solution sets consisting of:
- Solution Compound Buffer Precipitant Salt (a) Crystallization Solution Set 1:
- 1 100.000 mM HEPES/NaOH pH:
- 10.000% v/v 200.000 mM 7.5 isopropanol sodium chloride 2 100.000 mM Na3 citrate/citric acid 20.000% w/v pH:
- 5.5 PEG-3000 3 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-8000 ZnAc2 4 100.000 mM Na3 citrate/citric acid 2000.000 pH:
- 5.5 mM (NH4)2 sulfate 5 100.000 mM Tris base/HCl pH:
- 7. 20.000% w/v PEG-2000 MME 6 100.000 mM MES/NaOH pH:
- 6. 20.000% v/v 200.000 mM 1,4-butane- Li2 sulfate diol 7 100.000 mM imidazole/HCl pH:
- 8. 20.000% w/v 200.000 mM PEG-1000 CaAc2 8 100.000 mM Na2 H phosphate/K 2500.000 H2 phosphate pH:
- 6.2 mM sodium chloride 9 100.000 mM acetic acid/NaAc pH:
- 30.000% w/v 200.000 mM 4.5 PEG-8000 Li2 sulfate 10 100.000 mM Tris base/HCl pH:
- 10.000% v/v 8.5 isopropanol 11 100.000 mM Tris base/HCl pH:
- 30.000% v/v 200.000 mM 8.5 PEG-400 MgCl2 12 100.000 mM imidazole/HCl pH:
- 8. 2500.000 mM sodium chloride 13 100.000 mM MES/NaOH pH:
- 6. 10.000% v/v 200.000 mM isopropanol CaAc2 14 100.000 mM Tris base/HCl pH:
- 7. 15.000% v/v EtOH 15 100.000 mM acetic acid/NaAc pH:
- 30.000% v/v 200.000 mM 4.5 PEG-400 CaAc2 16 100.000 mM acetic acid/NaAc pH:
- 20.000% w/v 200.000 mM 4.5 PEG-1000 ZnAc2 17 100.000 mM acetic acid/NaAc pH:
- 10.000% w/v 200.000 mM 4.5 PEG-3000 ZnAc2 18 100.000 mM Na2 H phosphate/K 10.000% w/v 200.000 mM H2 phosphate pH:
- 6.2 PEG-8000 sodium chloride 19 100.000 mM Tris base/HCl pH:
- 1000.000 8.5 mM (NH4)2 H phosphate 20 100.000 mM Na cacodylate/HCl 10.000% v/v 200.000 mM pH:
- 6.5 isopropanol ZnAc2 21 100.000 mM Na cacodylate/HCl 30.000% v/v 200.000 mM pH:
- 6.5 PEG-400 Li2 sulfate 22 100.000 mM Na3 citrate/citric acid 15.000% v/v 200.000 mM pH:
- 5.5 EtOH Li2 sulfate 23 100.000 mM HEPES/NaOH pH:
- 1260.000 7.5 mM (NH4)2 sulfate 24 100.000 mM Tris base/HCl pH:
- 7. 2500.000 200.000 mM mM sodium MgCl2 chloride 25 100.000 mM MES/NaOH pH:
- 6. 15.000% v/v 200.000 mM EtOH ZnAc2 26 100.000 mM imidazole/HCl pH:
- 8. 10.000% v/v isopropanol 27 100.000 mM HEPES/NaOH pH:
- 15.000% v/v 200.000 mM 7.5 EtOH MgCl2 28 100.000 mM Na cacodylate/HCl 10.000% w/v 200.000 mM pH:
- 6.5 PEG-3000 MgCl2 29 100.000 mM MES/NaOH pH:
- 6. 20.000% w/v 200.000 mM PEG-8000 CaAc2 30 100.000 mM CHES/NaOH pH:
- 1260.000 200.000 mM 9.5 mM (NH4)2 sodium sulfate chloride 31 100.000 mM imidazole/HCl pH:
- 8. 20.000% v/v 200.000 mM 1,4-butane- ZnAc2 diol 32 100.000 mM Tris base/HCl pH:
- 7. 1000.000 200.000 mM mM Na3 sodium citrate chloride 33 100.000 mM HEPES/NaOH pH:
- 30.000% v/v 200.000 mM 7.5 PEG-400 sodium chloride 34 100.000 mM Tris base/HCl pH:
- 7. 10.000% w/v 200.000 mM PEG-8000 MgCl2 35 100.000 mM Na cacodylate/HCl 20.000% w/v 200.000 mM pH:
- 6.5 PEG-1000 MgCl2 36 100.000 mM imidazole/HCl pH:
- 8. 2500.000 200.000 mM mM sodium ZnAc2 chloride 37 100.000 mM Na2 H phosphate/K 40.000% v/v H2 phosphate pH:
- 6.2 EG 38 100.000 mM Tris base/HCl pH:
- 30.000% v/v 200.000 mM 8.5 PEG-200 (NH4)2 H phosphate 39 100.000 mM HEPES/NaOH pH:
- 10.000% v/v 50.000 mM 7.5 glycerol Li2 sulfate 30.000% v/v PEG-600 40 100.000 mM HEPES/NaOH pH:
- 40.000% v/v 7.5 EG 5.000% w/v PEG-3000 41 100.000 mM Tris base/HCl pH:
- 7. 40.000% v/v EG 42 100.000 mM Tris base/HCl pH:
- 50.000% v/v 200.000 mM 8.5 EG MgCl2 43 100.000 mM Tris base/HCl pH:
- 5.000% w/v 8.5 PEG-8000 10.000% v/v glycerol 20.000% v/v PEG-300 44 100.000 mM acetic acid/NaAc pH:
- 40.000% v/v 4.5 PEG-400 45 100.000 mM Tris base/HCl pH:
- 7. 10.000% v/v 500.000 mM glycerol (NH4)2 30.000% v/v sulfate PEG-600 46 100.000 mM HEPES/NaOH pH:
- 50.000% v/v 200.000 mM 7.5 EG Li2 sulfate 47 100.000 mM acetic acid/NaAc pH:
- 30.000% v/v 100.000 mM 4.5 PEG-200 sodium chloride 48 100.000 mM CAPS/NaOH pH:
- 30.000% v/v 200.000 mM 10.5 PEG-200 (NH4)2 sulfate (b) Crystallization Solution Set 2:
- 1 100.000 mM Tris base/HCl pH:
- 7. 10.000% v/v 200.000 mM isopropanol ZnAc2 2 100.000 mM acetic acid/NaAc pH:
- 10.000% v/v 200.000 mM 4.5 isopropanol CaAc2 3 100.000 mM Na3 citrate/Na3 10.000% v/v 200.000 mM citrate pH:
- 5.5 isopropanol Li2 sulfate 4 100.000 mM Tris base/HCl pH:
- 7. 10.000% v/v 200.000 mM isopropanol CaAc2 5 100.000 mM acetic acid/NaAc pH:
- 10.000% v/v 200.000 mM 4.5 isopropanol sodium chloride 6 100.000 mM Na3 citrate/Na3 10.000% v/v 200.000 mM citrate pH:
- 5.5 isopropanol MgCl2 7 100.000 mM HEPES/NaOH pH:
- 10.000% w/v 200.000 mM 7.5 PEG-8000 sodium chloride 8 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-8000 ZnAc2 9 100.000 mM Na2 H phosphate/K 10.000% w/v 200.000 mM H2 phosphate pH:
- 6.2 PEG-8000 Li2 sulfate 10 100.000 mM acetic acid/NaAc pH:
- 10.000% w/v 200.000 mM 4.5 PEG-8000 CaAc2 11 100.000 mM Tris base/HCl pH:
- 10.000% w/v 200.000 mM 8.5 PEG-8000 MgCl2 12 100.000 mM Na cacodylate/HCl 10.000% w/v 200.000 mM pH:
- 6.5 PEG-8000 sodium chloride 13 100.000 mM Na3 citrate/citric acid 10.000% w/v 200.000 mM pH:
- 5.5 PEG-8000 Li2 sulfate 14 100.000 mM HEPES/NaOH pH:
- 10.000% w/v 200.000 mM 7.5 PEG-8000 MgCl2 15 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 200.000 mM 7.5 PEG-2000 NaCl MME 16 100.000 mM MES/NaOH pH:
- 6. 20.000% w/v 200.000 mM PEG-2000 ZnAc2 MME 17 100.000 mM Na2 H phosphate/K 20.000% w/v H2 phosphate pH:
- 6.2 PEG-2000 MME 200.000 mM Li2 sulfate 18 100.000 mM acetic acid/NaAc pH:
- 20.000% w/v 200.000 mM 4.5 PEG-2000 CaAc2 MME 19 100.000 mM Tris base/HCl pH:
- 20.000% w/v 200.000 mM 8.5 PEG-2000 MgCl2 MME 20 100.000 mM cacodylic acid/Na 20.000% w/v cacodylate pH:
- 6.5 PEG-2000 MME 200.000 mM Li2 sulfate 21 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 7.5 PEG-2000 MME 200.000 mM Li2 sulfate 22 100.000 mM Tris base/HCl pH:
- 20.000% v/v 8.5 1,4-butane- diol 200.000 mM Li2 sul- fate 23 100.000 mM cacodylic acid/Na 20.000% v/v 200.000 mM cacodylate pH:
- 6.5 1,4-butane- CaAc2 diol 24 100.000 mM Na3 citrate/citric acid 20.000% v/v 200.000 mM pH:
- 5.5 1,4-butane- MgCl2 diol (c) Crystallization Solution Set 3:
- 1 100.000 mM Tris base/HCl pH:
- 7. 20.000% v/v 200.000 mM 1,4-butane- ZnAc2 diol 2 100.000 mM Tris base/HCl pH:
- 20.000% v/v 200.000 mM 8.5 1,4-butane- MgCl2 diol 3 100.000 mM Na3 citrate/citric acid 20.000% v/v 200.000 mM pH:
- 5.5 1,4-butane- sodium diol chloride 4 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 200.000 mM 7.5 PEG-1000 sodium chloride 5 100.000 mM MES/NaOH pH:
- 6 20.000% w/v 200.000 mM PEG-1000 ZnAc2 6 100.000 mM Tris base/HCl pH:
- 7. 20.000% w/v 200.000 mM PEG-1000 Li2 sulfate 7 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 200.000 mM 7.5 PEG-1000 MgCl2 8 100.000 mM MES/NaOH pH:
- 6. 20.000% w/v 200.000 mM PEG-1000 sodium chloride 9 100.000 mM HEPES/NaOH pH:
- 2500.000 7.5 mM sodium chloride 10 100.000 mM MES/NaOH pH:
- 6. 2500.000 200.000 mM mM sodium ZnAc2 chloride 11 100.000 mM Tris base/HCl pH:
- 2500.000 200.000 mM 8.5 mM sodium Li2 sulfate chloride 12 100.000 mM cacodylic acid/Na 2500.000 200.000 mM cacodylate pH:
- 6.5 mM sodium CaAc2 chloride 13 100.000 mM Na3 citrate/citric acid 2500.000 200.000 mM pH:
- 5.5 mM sodium MgCl2 chloride 14 100.000 mM cacodylic acid/Na 2500.000 200.000 mM cacodylate pH:
- 6.5 mM sodium Li2 sulfate chloride 15 100.000 mM Tris base/HCl pH:
- 7. 30.000% v/v 200.000 mM PEG-400 ZnAc2 16 100.000 mM imidazole/HCl pH:
- 8. 30.000% v/v 200.000 mM PEG-400 Li2 sulfate 17 100.000 mM Na3 citrate/citric acid 30.000% v/v 200.000 mM pH:
- 5.5 PEG-400 MgCl2 18 100.000 mM Na3 citrate/citric acid 30.000% v/v 200.000 mM pH:
- 5.5 PEG-400 Li2 sulfate 19 100.000 mM imidazole/HCl pH:
- 8. 30.000% v/v 200.000 mM PEG-400 ZnAc2 20 100.000 mM Tris base/HCl pH:
- 7. 30.000% v/v 200.000 mM PEG-400 NaCl 21 100.000 mM Na2 H phosphate/K 15.000% v/v 200.000 mM H2 phosphate pH:
- 6.2 EtOH sodium chloride 22 100.000 mM acetic acid/NaAc pH:
- 15.000% v/v 200.000 mM 4.5 EtOH ZnAc2 23 100.000 mM cacodylic acid/Na 15.000% v/v 200.000 mM cacodylate pH:
- 6.5 EtOH CaAc2 24 100.000 mM acetic acid/NaAc pH:
- 15.000% v/v 200.000 mM 4.5 EtOH MgCl2 (d) Crystallization Solution Set 4:
- 1 100.000 mM cacodylic acid/Na 15.000% v/v 200.000 mM cacodylate pH:
- 6.5 EtOH sodium chloride 2 100.000 mM HEPES/HEPES pH:
- 10.000% w/v 200.000 mM 7.5 PEG-3000 sodium chloride 3 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-3000 ZnAc2 4 100.000 mM Tris base/HCl pH:
- 10.000% w/v 200.000 mM 8.5 PEG-3000 CaAc2 5 100.000 mM Na3 citrate/citric acid 10.000% w/v 200.000 mM pH:
- 5.5 PEG-3000 MgCl2 6 100.000 mM Tris base/HCl pH:
- 10.000% w/v 200.000 mM 8.5 PEG-3000 Li2 sulfate 7 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-3000 Li2 sulfate 8 100.000 mM HEPES/HEPES pH:
- 1000.000 200.000 mM 7.5 mM (NH4)2 sodium H phosphate chloride 9 100.000 mM cacodylic acid/Na 1000.000 200.000 mM cacodylate pH:
- 6.5 mM (NH4)2 sodium H phosphate chloride 10 100.000 mM Na2 H phosphate/K 1260.000 200.000 mM H2 phosphate pH:
- 6.2 mM (NH4)2 Li2 sulfate sulfate 11 100.000 mM Tris base/HCl pH:
- 7. 1260.000 200.000 mM mM (NH4)2 MgCl2 sulfate 12 100.000 mM imidazole/HCl pH:
- 8. 1260.000 200.000 mM mM (NH4)2 Li2 sulfate sulfate 13 100.000 mM Na3 citrate/citric acid 1260.000 200.000 mM pH:
- 5.5 mM (NH4)2 sodium sulfate chloride 14 100.000 mM HEPES/HEPES pH:
- 20.000% w/v 200.000 mM 7.5 PEG-8000 sodium chloride 15 100.000 mM Tris base/HCl pH:
- 7. 20.000% w/v 200.000 mM PEG-8000 ZnAc2 16 100.000 mM imidazole/HCl pH:
- 8. 20.000% w/v 200.000 mM PEG-8000 Li2 sulfate 17 100.000 mM acetic acid/NaAc pH:
- 20.000% w/v 200.000 mM 4.5 PEG-8000 MgCl2 18 100.000 mM cacodylic acid/Na 20.000% w/v 200.000 mM cacodylate pH:
- 6.5 PEG-8000 sodium chloride 19 100.000 mM Tris base/HCl pH:
- 7. 20.000% w/v 200.000 mM PEG-8000 MgCl2 20 100.000 mM HEPES/HEPES pH:
- 1000.000 200.000 mM 7.5 mM Na3 sodium citrate chloride 21 100.000 mM MES/NaOH pH:
- 6. 1000.000 200.000 mM mM Na3 ZnAc2 citrate 22 100.000 mM imidazole/HCl pH:
- 8. 1000.000 200.000 mM mM Na3 Li2 sulfate citrate 23 100.000 mM acetic acid/NaAc pH:
- 1000.000 200.000 mM 4.5 mM Na3 MgCl2 citrate 24 100.000 mM Tris base/HCl pH:
- 1000.000 200.000 mM 8.5 mM Na3 Li2 sulfate citrate (e) Crystallization Solution Set 5:
- 1 100.000 mM MES/NaOH pH:
- 6. 1000.000 200.000 mM mM Na3 MgCl2 citrate 2 100.000 mM acetic acid/NaAc pH:
- 10.000% v/v 200.000 mM 4.5 isopropanol Li2 sulfate 3 100.000 mM Na3 citrate/citric acid 10.000% v/v 200.000 mM pH:
- 5.5 isopropanol sodium chloride 4 100.000 mM acetic acid/NaAc pH:
- 10.000% w/v 200.000 mM 4.5 PEG-8000 MgCl2 5 100.000 mM Tris base/HCl pH:
- 10.000% w/v 200.000 mM 8.5 PEG-8000 sodium chloride 6 100.000 mM acetic acid/NaAc pH:
- 20.000% w/v 200.000 mM 4.5 PEG-2000 ZnAc2 MME 7 100.000 mM Tris base/HCl pH:
- 20.000% w/v 200.000 mM 8.5 PEG-2000 Li2 sulfate MME 8 100.000 mM Tris base/HCl pH:
- 20.000% w/v 200.000 mM 8.5 1,4-butane- sodium diol chloride 9 100.000 mM cacodylic acid/Na 20.000% w/v 200.000 mM cacodylate pH:
- 6.5 1,4-butane- Li2 sulfate diol 10 100.000 mM HEPES/NaOH pH:
- 20.000% w/v 200.000 mM 7.5 PEG-1000 Li2 sulfate 11 100.000 mM MES/NaOH pH:
- 6. 20.000% w/v 200.000 mM PEG-1000 MgCl2 12 100.000 mM MES/NaOH pH:
- 6. 2500.000 200.000 mM mM sodium Li2 sulfate chloride 13 100.000 mM Tris base/HCl pH:
- 30.000% w/v 200.000 mM 8.5 PEG-8000 Li2 sulfate 14 100.000 mM cacodylic acid/Na 30.000% w/v 200.000 mM cacodylate pH:
- 6.5 PEG-8000 CaAc2 15 100.000 mM MES/NaOH pH:
- 6. 30.000% w/v 200.000 mM PEG-400 MgCl2 16 100.000 mM Tris base/HCl pH:
- 7. 30.000% w/v 200.000 mM PEG-400 Li2 sulfate 17 100.000 mM HEPES/NaOH pH:
- 10.000% w/v 200.000 mM 7.5 PEG-3000 Li2 sulfate 18 100.000 mM MES/NaOH pH:
- 6. 10.000% w/v 200.000 mM PEG-3000 MgCl2 19 100.000 mM imidazole/HCl pH:
- 8. 1000.000 200.000 mM mM Na3 MgCl2 citrate 20 100.000 mM Tris base/HCl pH:
- 1000.000 200.000 mM 8.5 mM Na3 sodium citrate chloride 21 100.000 mM Tris base/HCl pH:
- 7. 2500.000 mM NaMal 22 100.000 mM acetic acid/NaAc pH:
- 2500.000 4.5 mM NaMal 23 100.000 mM Tris base/HCl pH:
- 2500.000 8.5 mM NaMal 24 100.000 mM imidazole/HCl pH:
- 8. 2500.000 mM NaMal.
147 more elements
Dependencies are inferred from the claim text.