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US 6,596,850 B1Granted
Anti-αv3β3 recombinant human antibodies, nucleic acids encoding same
AssigneeIxsys, Inc.
InventorWilliam D. Huse
Life of this patent
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AssigneeIxsys, Inc.
InventorWilliam D. Huse
Estimating the term…
The invention provides a LM609 grafted antibody comprising one or more CDRs having at least one amino acid substitution, where the LM609 grafted antibody has αvβ3 binding activity. Nucleic acids encoding LM609 grafted heavy and light chains are additionally provided. Functional fragments of such encoding nucleic acids are similarly provided. The invention also provides a method of inhibiting a function of αvβ3. The method consists of contacting αvβ3 with a LM609 grafted antibody or functional fragments thereof under conditions which allow binding to αvβ3. Finally, the invention provides for a method of treating an αvβ3-mediated disease. The method consists of administering an effective amount a LM609 grafted antibody or functional fragment thereof under conditions which allow binding to αvβ3.
A high affinity LM609 grafted antibody exhibiting selective binding affinity to αvβ3, or a functional fragment thereof, comprising one or more CDRs having at least one amino acid substitution in one or more CDRs of a LM609 grafted heavy chain variable region polypeptide referenced as SEQ ID NO:6 or a LM609 grafted light chain variable region polypeptide referenced as SEQ ID NO:8, said antibody or functional fragment thereof having integrin αvβ3 binding activity, integrin αvβ3 binding specificity or integrin αvβ3-inhibitory activity, wherein the αvβ3 binding affinity of said high affinity LM609 grafted antibody is higher affinity relative to parental LM609 grafted antibody.
An antibody, or a functional fragment thereof, comprising one or more CDRs selected from the group consisting of SEQ ID NO:48, SEQ ID NO:50, SEQ ID NO:52, SEQ ID NO:54, SEQ ID NO:56, SEQ ID NO:58, SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:64, SEQ ID NO:66, SEQ ID NO:68, SEQ ID NO:70, SEQ ID NO:72, SEQ ID NO:74, SEQ,ID NO:76, SEQ ID NO:78, SEQ ID NO:80, SEQ ID NO:82, SEQ ID NO:84, SEQ ID NO:86, SEQ ID NO:88, SEQ ID NO:90, SEQ ID NO:92, SEQ ID NO:94, SEQ ID NO:96;
A grafted antibody exhibiting selective binding affinity to αvβ3, or a functional fragment thereof, comprising one or more CDRs having at least one amino acid substitution in one or more CDRs of the heavy chain variable region polypeptide referenced as SEQ ID NO:6 or the light chain variable region polypeptide referenced as SEQ ID NO:8, said antibody or functional fragment thereof having increased integrin αvβ3 binding activity, integrin αvβ3 binding specificity or integrin αvβ3-inhibitory activity relative to parental antibody comprising a heavy chain variable region polypeptide referenced as SEQ ID NO:6 and a light chain variable region amino acid sequence referenced as SEQ ID NO:8.
Dependencies are inferred from the claim text.