Monoclonal-antibody for analysis and clearance of polyethylene glycol and polyethylene glycol-modified molecules
AssigneeAcademia Sinica
InventorsSteve Roffler, Tian-Lu Cheng, Pin-Yi Wu
Life of this patent
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Abstract
A novel anti-polyethylene glycol monoclonal antibody and its preparation are disclosed. Such an antibody can be used for determining polyethylene glycol concentration in vitro or accelerating the clearance of a polyethylene glycol containing compound from the blood circulation in the human body thereby reducing the toxicity associated with the polyethylene glycol containing conjugate. The antibody is particularly useful in cancer therapy where the therapeutic agent is selectively delivered to the tumor by increasing the tumor/blood ratio of the polyethylene glycol containing compound.
Claims18
A monoclonal antibody AGP3 which is produced by hybridoma having deposit number CCTCC-V-200001.
A method of producing monoclonal antibody AGP3 produced from a hybridoma having deposit number CCTCC-V-200001, comprising the steps of:
- a) producing an immunogenic compound comprising a polyethylene glycol moiety and an immunogenic moiety;
- b) preparing an injectable solution comprising said immunogenic compound in phosphate buffered saline (PBS);
- c) immunizing a mouse with said injectable solution by a combination of intravenous and intraperitoneal injections,
- d) producing a hybridoma by fusing a spleen cell from said immunized mouse with a myeloma cell;
- e) selecting a hybridoma producing a monoclonal antibody AGP3 which binds to a polyethylene glycol molecule or a polyethylene glycol moiety of a polyethylene-glycol-containing compound;
- and
- f) isolating said monoclonal antibody.
A monoclonal antibody AGP3 produced by a hybridoma having deposit number CCTCC-V-200001 which binds to an eiltope comprising —[OCH2CH2]— a moiety.
A hybridoma having deposit number CCTCC C 200205.
A method for identifying or measuring the concentration of a polyethylene glycol or a polyethylene-glycol-containing compound, comprising the steps of:
- a) obtaining a sample to be identified;
- b) contacting monoclonal antibody AGP3 produced from a hybridoma having deposit number CCTCC-V-2000001 with said sample under the conditions for polyethylene glycol binding for a period of time sufficient to allow binding of said monoclonal antibody to said polyethylene glycol or said polyethyleneglycol-containing compound and measuring the amount of said polyethylene glycol or said polyethylene-glycol-containing compound bound to said monoclonal antibody.
A method for identifying or measuring the concentration of a polyethylene glycol or a polyethylene-glycol-containing compound, comprising the steps of:
- a) coating a solid support with monoclonal antibody AGP3 produced from a hybridoma having deposit number CCTCC-V-200001;
- b) contacting said monoclonal antibody on said solid support with a polyethylene glycol or a polyethylene-glycol-containing compound, thereby capturing said polyethylene glycol or said polyethylene-glycol-containing compound;
- c) contacting said captured polyethylene glycol or polyethylene-glycol-containg compound with said monoclonal antibody that has been radiolabeled, linked to an enzyme or biotinylated;
- and
- d) measuring the amount of said bound antibody.
Dependencies are inferred from the claim text.