Furin polypeptides with improved characteristics
AssigneeBaxter Aktiengesellschaft
InventorsBarbara Plaimauer, Uwe Schlokat
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Abstract
The present invention comprises a furin polypeptide having a modified amino acid sequence between the middle, homo-B-domain and the transmembrane domain compared to wild-type furin which retains proteolytic activity but is secreted at lower levels in cell culture compared to wild-type furin. Additionally, the invention includes nucleic acid molecules encoding such furin polypeptides, vectors and host cells comprising said nucleic acid molecules, compositions comprising said furin polypeptide and methods for producing such compositions.
Claims15
A furin polypeptide comprising amino acids, said amino acids having a sequence which comprises a modification compared to the amino acid sequence of wild-type furin as set forth in SEQ ID NO:1, wherein said modification is present between amino acids Ala 557 and Leu713 inclusive, of wild-type furin, and further wherein said modification results in the formation of a loop or alpha-helix structure between amino acids Ala 557 and Leu713 of wild-type furin.
Dependencies are inferred from the claim text.