Immobilizing lipase by adsorption from a crude solution onto nonpolar polyolefin particles
AssigneeBASF SE
InventorsThomas Friedrich, Rainer Stürmer
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Abstract
Immobilized lipase is prepared by adsorbing lipase from a crude lipase solution onto polyolefin particles such as polypropylene particles which are nonpolar. The crude solution may be a cell-free culture broth. Lipase sources include Pseudomonas burkholderia and Pseudomonas aeruginosa. Uses of the immobilized lipase include enantioselective conversion of substrates such as enantioselective acylating or hydrolyzing.
Claims10
A process for preparing immobilized lipase consisting essentially of contacting a crude lipase solution with nonpolar polyolefin particles to absorb lipase in the solution to the particles, wherein said crude lipase solution contains more than 2% by weight of impurities comprising proteins other than lipase, cellular constituents of a lipase-producing microorganism and or nutrient media residues.
Dependencies are inferred from the claim text.