Back to results
US 6,596,491 B2Granted
Tenascin-C nucleic acid ligands
AssigneeGilead Sciences, Inc.
InventorsBrian Hicke, Stephen Warren, David Parma, Larry Gold
Life of this patent
Estimating the term…
Abstract
Methods are described for the identification and preparation of nucleic acid ligands to tenascin-C. Included in the invention are specific RNA ligands to tenascin-C identified by the SELEX method. Further included in the invention are methods for detecting the presence of a disease condition in a biological tissue in which tenascin-C is expressed.
Claims18
A complex for use in in vivo diagnostics comprising a tenascin-C nucleic acid ligand and a marker.
A method for the preparation of a complex comprised of a tenascin-C nucleic acid ligand and a marker, said method comprising:
- a) contacting a candidate mixture of nucleic acids with tenascin-C, wherein nucleic acids having an increased affinity to tenascin-C relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
- b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
- c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to tenascin-C to yield a tenascin-C nucleic acid ligand;
- and
- d) covalently linking said tenascin-C nucleic acid ligand with a marker.
2 more elements
Dependencies are inferred from the claim text.