Density gradient solutions of metal ion chelate complexes
AssigneeThe Texas A&M University System
InventorsRonald D. Macfarlane, Brian D. Hosken
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Abstract
Methods of forming a density gradient by applying a centrifugal field to a solution of one or more metal ion chelate complexes are disclosed. The density gradients are self-forming equilibrium gradients and are useful for separating biological particles by ultracentrifugation. Also disclosed are methods of separating biological particles according to their density. Also disclosed are density gradients of lipoprotein particles and one or more metal ion chelate complexes, wherein the lipoprotein particles are partitioned along the density gradient according to their particle density.
Claims31
A method of forming a density gradient, the method comprising:
- applying a centrifugal field to a solution of one or more metal ion chelate complexes until the one or more metal ion chelate complexes forms a density gradient.
A method of separating particles according to their density, the method comprising:
- providing a composition comprising the particles and a solution of one or more metal ion chelate complexes;
- and
- applying a centrifugal field to he composition until the one or more metal ion chelate complexes form a density gradient and the particles have partitioned along the density gradient according to their density.
A density gradient formed from one or more metal ion chelate complexes.
A composition comprising a density gradient of one or more metal ion chelate complexes and lipoprotein particles, wherein the lipoprotein particles are partitioned along the density gradient according to the density of the lipoprotein particles.
Dependencies are inferred from the claim text.