Enhancing contrast in biological imaging
AssigneeBoard of Regents, The University of Texas System
InventorsRebecca R. Richards-Kortum, Andrés F. Zuluaga, Rebekah Drezek, Colin Smithpeter, Tom Collier
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Abstract
Methods for enhancing contrast during imaging to assess cell and nuclear morphology of a sample. A contrast agent such as acetic acid, toluidine blue, hypertonic saline, hypotonic saline, Lugol's iodine, an absorbing dye, a liposome, or a contrast agent linked to a marker are applied to a sample. The sample is analyzed with an imaging device to create image data, and the sample is diagnosed using the image data.
Claims38
A method for enhancing contrast during imaging to assess cell and nuclear morphology of a sample, comprising:
- applying between about 0.5% and about 10% by volume of toluidine blue to the sample;
- analyzing the sample with a confocal microscope to create image data;
- and
- diagnosing the sample with the image data.
A method for enhancing contrast during imaging to enhance edges of cells of a sample, comprising:
- applying between about 2 to about 10 times physiological concentrations of hypertonic saline to the sample;
- analyzing the sample with an imaging device to create image data;
- and
- diagnosing the sample with the image data.
A method for enhancing contrast during imaging to enhance edges of cells of a sample, comprising:
- applying between about 0.1 to about 0.5 times physiological concentrations of hypotonic saline to the sample;
- analyzing the sample with an imaging device to create image data;
- and
- diagnosing the sample with the image data.
A method for enhancing contrast during imaging to assess cell and nuclear morphology of a sample, comprising:
- applying between about 5% and about 10% by volume of Lugol's iodine to the sample;
- analyzing the sample with a confocal microscope to create image data;
- and
- diagnosing the sample with the image data.
A method for enhancing contrast during imaging to assess cell and nuclear morphology of a sample, comprising:
- applying an absorbing dye comprising phycoerythrin or lutetium texaphyrin to the sample;
- analyzing the sample with an imaging device to create image data;
- and
- diagnosing the sample with the image data.
A method for enhancing contrast during imaging to assess cell and nuclear morphology of a sample, comprising:
- applying a liposome to the sample, the liposome containing a fluid of different refractive index;
- analyzing the sample with an imaging device to create image data;
- and
- diagnosing the sample with the image data.
A method for enhancing contrast during imaging to assess cell and nuclear morphology of a sample, comprising:
- applying a contrast agent linked to a marker to the sample;
- analyzing the sample with an imaging device to create image data;
- and
- diagnosing the sample with the image data.
A method for enhancing contrast during imaging to assess cell and nuclear morphology of a sample, comprising:
- applying between about 0.5% and about 10% by volume of toluidine blue to the sample;
- analyzing the sample with an imaging device to create image data, the imaging device comprising an optical coherence tomography apparatus, a photon migration imaging device, a two-photon excited fluorescence imaging device, an absorption spectroscopy apparatus, or a Raman spectroscopy apparatus;
- and
- diagnosing the sample with the image data.
A method for enhancing contrast during imaging to assess cell and nuclear morphology of a sample, comprising:
- applying between about 5% and about 10% by volume of Lugol's iodine to the sample;
- analyzing the sample with an imaging device to create image data;
- the imaging device comprising an optical coherence tomography apparatus, a photon migration imaging device, a two-photon excited fluorescence imaging device, an absorption spectroscopy apparatus, or a Raman spectroscopy apparatus;
- and
- diagnosing the sample with the image data.
Dependencies are inferred from the claim text.