Method and kit using recombinant proteins in fusion of porcine reproductive and respiratory syndrome virus and for diagnosis
AssigneeInmunologia Y Genetica Aplicada, S. A.
InventorsMa Jose Rodriguez Garcia, Antonio Sanz Fernandez, Jose Ignacio Casal Alvarez
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Abstract
The recombinant fusion proteins comprise the protein of the nucleocapsid of the porcine reproductive and respiratory syndrome virus (PRRSV) obtained both from a European isolate of PRRSV as well as from an American isolate, fused to a sequence of amino acids derived from the system chosen for the expression of said recombinant fusion protein, either directly or by means of a binding area formed by a small number of amino acids. These recombinant fusion proteins are suited for their use in the diagnosis of PRRSV.
Claims2
A method for the diagnosis of porcine reproductive and respiratory syndrome virus (PRRSV) comprising:
- contacting a biological sample from an animal with a recombinant fusion protein which comprises a sequence of amino acids from porcine reproductive and respiratory syndrome virus (PRRSV) selected between SEQ. ID. No.:
- 1 and SEQ. ID. No.:
- 2, and a sequence of 259 amino acids extending from amino acid residue number 1 to amino acid residue number 259 of the amino acid sequence shown in SEQ. ID. No.:8 or in SEQ. ID. No.:9, protein under conditions that allow formation of an antigen-antibody complex and
- detecting the complex formed.
Dependencies are inferred from the claim text.