Intramolecularly-quenched near infrared flourescent probes
AssigneeThe General Hospital Corporation
InventorsRalph Weissleder, Ching-Hsuan Tung, Umar Mahmood, Lee Josephson, Alexei Bogdanov
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Abstract
An intramolecularly-quenched, near infrared fluorescence probe that emits substantial fluorescence only after interaction with a target tissue (i.e., activation) is disclosed. The probe includes a polymeric backbone and a plurality of near infrared fluorochromes covalently linked to the backbone at fluorescence-quenching interaction-permissive positions separable by enzymatic cleavage at fluorescence activation sites. The probe optionally includes protective chains or fluorochrome spacers, or both. Also disclosed are methods of using the intramolecularly-quenched, near infrared fluorescence probes for in vivo optical imaging.
Claims208
An activatable imaging probe comprising a fluorochrome attachment moiety and a plurality of near-infrared fluorochromes linked to the fluorochrome attachment moiety at fluorescence-quenching interaction-permissive positions, wherein the probe is activatable by cleavage at fluorescence activation sites.
An activatable imaging probe comprising a fluorochrome attachment moiety and a plurality of fluorochromes linked to the fluorochrome attachment moiety at fluorescence-quenching interaction-permissible positions, wherein at least one of the fluorochromes is a near infrared fluorochrome and at least one of the other fluorochromes is an energy acceptor, and wherein the probe is activatable by cleavage at fluorescence activation sites.
An activatable imaging probe comprising a fluorochrome attachment moiety and a plurality of near-infrared fluorochromes linked to the fluorochrome attachment moiety at fluorescence-quenching interaction-permissive positions, wherein the probe is activatable by cleavage at fluorescence activation sites located in the fluorochrome attachment moiety.
An activatable imaging probe comprising a fluorochrome attachment moiety of methoxypoly(ethylene)glycol-succinyl-N-ε-poly-L-lysine and a plurality of fluorochromes linked to the ε-amino groups of the poly-L-lysine side chains at fluorescence-quenching interaction-permissible positions, wherein the probe is activatable by enzymatic cleavage at KK activation sites located in the fluorochrome attachment moiety.
An activatable imaging probe comprising a fluorochrome attachment moiety and a plurality of fluorochromes linked to the fluorochrome attachment moiety at fluorescence-quenching interaction-permissible positions, wherein at least one of the fluorochromes is a near infrared fluorochrome and at least one of the other fluorochromes is an energy acceptor, and wherein the probe is activatable by cleavage at fluorescence activation sites located in the fluorochrome attachment moiety.
An activatable imaging probe comprising a fluorochrome attachment moiety and a plurality of fluorochromes linked to the fluorochrome attachment moiety at fluorescence-quenching interaction-permissible positions, wherein the probe is activatable by enzymatic cleavage by a Cathepsin at activation sites located in the fluorochrome attachment moiety.
An activatable imaging probe comprising a fluorochrome attachment moiety and a plurality of near-infrared fluorochromes linked to the fluorochrome attachment moiety at fluorescence-quenching interaction-permissive positions through spacers, wherein the probe is activatable by cleavage at fluorescence activation sites located in the spacers.
An activatable imaging probe comprising a fluorochrome attachment moiety and a plurality of fluorochromes linked to the fluorochrome attachment moiety through spacers at fluorescence-quenching interaction-permissible positions, wherein at least one of the fluorochromes is a near infrared fluorochrome and at least one of the other fluorochromes is an energy acceptor, and wherein the probe is activatable by cleavage at fluorescence activation sites located in the spacer.
Dependencies are inferred from the claim text.