Multiple microchannels chip for biomolecule imaging, and method of use thereof

A method of observation by flat surface laser scanner of biological samples in a glass panel, the glass panel of the type defining a top flat surface, a bottom bearing surface, and a plurality of channels extending generally parallel to each other from said top to bottom surfaces and each defining a top access mouth for the biological sample, the method including the following steps:

• a) Inclining the channels in an oblique fashion so as to make a significant acute angle relative to an axis perpendicular to the top flat surface of the glass panel,
• b) Enlarging the inner diameter of each said channel enough as to accommodate power assisted flow through viscosity of a biological sample containing fluid;
• c) Providing fluorescein dyes inside the biological sample containing fluid;
• d) Directing a coherent laser beam transversely through a selected channel top access mouth and coaxially into the corresponding channel, so as to excite the fluorescein dyes, wherein an optically apparent glow is generated by the excited dyes without a halo being generated about the top access mouth;
• e) Allowing sufficient time for the fluorescein dyes to project the optically apparent glow upwardly beyond said channel top access mouth;
• and
• f) Performing optical measurements of this upward out of channel projecting fluorescein glow to generate evidence data on the chemical properties and location of the biological samples.

A method of observation by flat surface laser scanner of biological samples in a glass panel, the glass panel of the type defining a top flat surface, a bottom bearing surface, and a plurality of channels extending generally parallel to each other from said top to bottom surfaces and each defining a top access mouth for the biological sample, the method including the following steps:

• a) Inclining the channels in an oblique fashion so as to make a significant acute angle relative to an axis perpendicular to the top flat surface of the glass panel,
• b) Enlarging the inner diameter of each said channel enough as to accommodate power assisted flow through viscosity of a biological sample containing fluid;
• c) Directing a coherent laser beam transversely through a selected channel top access mouth and coaxially into the corresponding channel;
• d) Measuring deterioration of light intensity upon the binding of biomolecules to the channels inner wall;
• and
• e) Performing analysis of this light intensity deterioration to generate evidence data on the chemical properties and location of the biological samples.