Soybean peroxidase gene family and an assay for detecting soybean peroxidase activity
AssigneeIndiana Crop Improvement Association
InventorRichard A. Vierling, Jr.
Life of this patent
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Abstract
The present invention relates to the genomic DNA and promoters of soybean peroxidases and their use as promoters for producing transgenic plants, including transgenic soybeans. The invention also relates to immunoassays or oligouncleotide assays which utilize soybean peroxidase as a marker. The invention further relates to the use of third antibody, an anti-soybean peroxidase antibody, in immunoassays. Soybean peroxidase may be bound to the anti-soybean peroxidase antibody prior to binding of this antibody with the second antibody (anti-antibody) in the assay. Alternatively, the anti-soybean peroxidase antibody is bound to the second antibody (anti-antibody) and then the soybean peroxidase bound by its specific antibody.
Claims27
A recombinant DNA molecule comprising:
- a) a GmEPC regulatory sequence consisting of a nucleotide sequence consisting of nucleotides:
- 1326 to 1532 in SEQ ID NO:20 in combination with a heterologous plant expressible promoter;
- and
- b) a heterologous structural gene,
- wherein said heterologous structural gene is under the regulatory control of said regulatory sequence and said plant expressible promoter.
A recombinant promoter molecule, said promoter molecule comprising:
- a) a GmEPC regulatory sequence consisting of nucleotide sequence:
- 1326 to 1532 in SEQ ID NO:20;
- b) a heterologous plant expressible promoter, providing a TATA box region and a transcription start site positioned 3′ to said regulatory sequence,
- whereby a heterologous plant expressible structural gene, placed 3′ to said recombinant promoter molecule is expressed under the regulatory control of said recombinant promoter molecule.
A recombinant promoter molecule, said promoter molecule comprising a truncated, plant expressible promoter, providing a TATA box region and a transcription start site, whereby a heterologous plant expressible structural gene, placed 3′ to said recombinant promoter molecule is expressed under the regulatory control of said recombinant promoter molecule, and wherein said truncated plant expressible promoter is selected from the group consisting of:
- a) a nucleotide sequence consisting of 9 to 1532 in SEQ ID NO:20;
- b) a nucleotide sequence consisting of 459 to 1532 in SEQ ID NO:20;
- c) a nucleotide sequence consisting of 1066 to 1532 in SEQ ID NO:20;
- and
- d) a nucleotide sequence consisting of 1326 to 1532 in SEQ ID NO:20.
A recombinant DNA molecule comprising:
- a) a GmEPC regulatory sequence consisting of the nucleotide sequence from nucleotides 1326 to 1532 of SEQ ID NO:
- 20;
- and
- b) a heterologous structural gene,
- wherein said heterologous structural gene is under the regulatory control of said regulatory sequence.
A recombinant DNA molecule comprising:
- a) a GmEPC regulatory sequence selected from the group consisting of:
- i) a nucleotide sequence consisting of nucleotides 1066 to 1532 in SEQ ID NO:20;
- and
- ii) a nucleotide sequence consisting of nucleotides 1326 to 1532 in SEQ ID NO:20 in combination with a heterologous plant expressible promoter;
- and
- b) a heterologous structural gene,
- wherein said heterologous structural gene is under the regulatory control of said regulatory sequence and said plant expressible promoter.
A recombinant promoter molecule, said promoter molecule comprising:
- a) a GmEPC regulatory sequence selected from the group consisting of:
- i) a nucleotide sequence consisting of nucleotides 1066 to 1532 in SEQ ID NO:20;
- and
- ii) a nucleotide sequence consisting of nucleotides 1326 to 1532 in SEQ ID NO:20;
- b) a heterologous plant expressible promoter, providing a TATA box region and a transcription start site positioned 3′ to said regulatory sequence,
- whereby a heterologous plant expressible structural gene, placed 3′ to said recombinant promoter molecule is expressed under the regulatory control of said recombinant promoter molecule.
A recombinant DNA molecule consisting of:
- a) a GmEPC regulatory sequence selected from the group consisting of:
- i) a nucleotide sequence consisting of nucleotides 1066 to 1532 in SEQ ID NO:20;
- and
- ii) a nucleotide sequence consisting of nucleotides 1326 to 1532 in SEQ ID NO:20;
- and
- b) a heterologous structural gene,
- wherein said heterologous structural gene is under the regulatory control of said regulatory sequence.
A recombinant promoter molecule, said promoter molecule consisting of:
- a) a GmEPC regulatory sequence consisting of the nucleotide sequence from nucleotides 1326 to 1532 of SEQ ID NO:
- 20;
- and
- b) a transcription start site positioned 3′ to said regulatory sequence,
- whereby a heterologous plant expressible structural gene, placed 3′ to said recombinant promoter molecule is expressed under the regulatory control of said recombinant promoter molecule.
A recombinant promoter molecule, said promoter molecule consisting of:
- a) a GmEPC regulatory sequence selected from the group consisting:
- i) a nucleotide sequence consisting of nucleotides 1066 to 1532 in SEQ ID NO:20;
- and
- ii) a nucleotide sequence consisting of nucleotides 1326 to 1532 in SEQ ID NO:20;
- and
- b) a transcription start site positioned 3′ to said regulatory sequence,
- whereby a heterologous plant expressible structural gene, placed 3′ to said recombinant promoter molecule is expressed under the regulatory control of said recombinant promoter molecule.
Dependencies are inferred from the claim text.