Defl
AssigneeSmithKline Beecham Corporation
InventorsMichael Arthur Lonetto, Richard Lloyd Warren, Daniel Robert Sylvester
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Abstract
The invention provides Defl polypeptides and DNA (RNA) encoding Defl polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing Defl polypeptides to screen for antibacterial compounds.
Claims22
An isolated polynucleotide segment comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence;
- wherein the polynucleotide sequence is at least 95% identical to SEQ ID NO:1;
- wherein the isolated polynucleotide segment detects Staphylococcus aureus, and wherein the polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence is not genomic DNA.
An isolated polynucleotide segment comprising SEQ ID NO:1.
An isolated polynucleotide segment, comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence encodes the same polypeptide, expressed by the Defl gene encoded by a nucleic acid sequence comprising SEQ ID NO:1 contained in NCIMB Deposit No. 40771;
- wherein the polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence is not genomic DNA.
An isolated polynucleotide comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence;
- wherein the polynucleotide sequence hybridizes to the full complement of SEQ ID NO:1;
- wherein the hybridization conditions comprise incubation at 42° C. in a solution comprising:
- 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1×SSC at 65° C.;
- wherein the polynucleotide sequence is at least 95% identical to SEQ ID NO:1;
- wherein the isolated polynucleotide segment detects Staphylococcus aureus, and wherein the polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence is not genomic DNA.
An isolated polynucleotide segment encoding a polypeptide comprising SEQ ID NO:2.
An isolated polynucleotide segment encoding a polypeptide consisting of SEQ ID NO:2.
Dependencies are inferred from the claim text.