Purification of somatotropin from transformed microorganisms
AssigneePharmacia & Upjohn Company
InventorsTimothy W. Evans, Mark W. Knuth
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Abstract
This invention discloses a commercially advantageous process for extraction and purification of protein from microorganisms. The initial steps of the process are useful for purifying many insoluble proteins while later steps are designed to renature denatured somatotropins produced by transformed microorganisms. The process is especially useful for purifying recombinantly-produced bovine somatotropin.
Claims13
A process for oxidizing insoluble forms of somatotropin from transformed microorganisms to the native disulphide bond conformation which comprises oxidizing the somatotropin in the presence of a detergent selected from the group consisting of sodium dodecyl sulfate and compounds of the formula:
- wherein n is 8 through 20, inclusive;
- R1 is methyl or ethyl, and R2 is hydrogen, methyl, ethyl, isopropyl or n-propyl.
Dependencies are inferred from the claim text.