Microchip based enzymatic analysis

A method for the detection of cell contents, the method comprising the steps of:

• introducing a stream of multiple cells into a channel in a microchip;
• individually isolating a cell from the stream of multiple cells in a lysing zone and lysing the cell by application of an electric field across the cell in the lysing zone to release cell contents into the channel;
• moving the cell contents towards a detection zone;
• and
• detecting the cell contents at the detection zone.

A method for the detection of cell contents, the method comprising the steps of:

• introducing a stream of multiple cells into a channel in a microchip;
• individually isolating a cell from the stream of multiple cells in a lysing zone and lysing the cell to release cell contents into the channel at the lysing zone;
• reacting the cell contents with a substrate at a reaction zone downstream of the lysing zone to yield a reaction product;
• moving the reaction product towards a detection zone;
• and
• detecting the reaction product at the detection zone.

A method for the detection of cell contents, the method comprising the steps of:

• introducing a stream of multiple cells into a channel in a microchip;
• individually isolating a cell from the stream of multiple cells in a lysing zone and lysing the cell by application of an electric field across the cell in the lysing zone to release cell contents into the channel;
• reacting the cell contents with a substrate to create reaction products;
• moving the reaction products towards a detection zone;
• and
• detecting the reaction products at the detection zone.