Method for more efficient electroporation
AssigneeStratagene
InventorsAlan L. Greener, Bruce D. Jerpseth
Life of this patent
Estimating the term…
Abstract
This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.
Claims36
A method for transferring nucleic acids of interest into:
- competent host cells, comprising the steps of:
- (a) mixing competent host cells suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative with the nucleic acids of interest;
- and
- (b) subjecting the host cells to an electrical treatment, thereby permitting the transfer of the nucleic acids of interest into the bacterial cells.
The method of 1, further comprising the step of culturing the transformed cells in a selected media capable of promoting their growth.
An electroporation kit comprising transformation competent cells suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative.
Dependencies are inferred from the claim text.