Methods to identify compounds affecting mitochondria
AssigneeThe Johns Hopkins University
InventorsEduardo Marban, Brian O'Rourke
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Abstract
The present invention relates to methods for identifying a compound capable of modulating mitochondrial function, comprising contacting a eukaryotic cell with one or more candidate compounds, and detecting a change in the mitochondrial redox state of the cell. The methods further relates to such methods wherein endogenous fluorescence of the cell mitochondria is indicative of a change of redox state.
Claims3
A method for identifying a compound capable of increasing or decreasing the redox state of mitochondria, comprising:
- (a) contacting a eukaryotic cell with one or more candidate compounds, and
- (b) measuring fluorescence of an endogenous mitochondrial component;
- (c) detecting a change in fluorescence upon contact with a candidate compound, wherein a change in fluorescence is indicative of an increase or decrease in the mitochondrial redox state of the cell;
- and
- (d) selecting a compound or compounds which causes a change in fluorescence, wherein the candidate compound activates a mitochondrial KATP channel.
A method for identifying a compound capable of increasing or decreasing the redox state of mitochondria, comprising.
- (c) contacting a eukaryotic cell with one or more candidate compounds, and
- (b) measuring fluorescence of an endogenous mitochondrial component;
- (c) detecting a change in fluorescence upon contact with a candidate compound, wherein a change in fluorescence is indicative of an increase or decrease in the mitochondrial redox state of the cell;
- and
- (d) selecting a compound or compounds which causes a change in fluorescence;
- wherein the oxidation state of the endogenous mitochondrial flavin moiety is monitored by fluorescence, and an increase in fluorescence indicates a increased oxidation state and a reduced fluorescence indicates an reduced oxidations.
Dependencies are inferred from the claim text.