Recombinant PR-3 and compositions thereof
AssigneeChiron Corporation
InventorsRobert F. Halenbeck, Michael Kriegler, Carl Perez, David A. Jewell, Kirston E. Koths
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Abstract
Methods and materials are disclosed for the production of purified, active recombinant human neutrophil protease, PR-3, via activation of a pro-form herein referred to as proPR-3. Human PR-3 is useful for discovering inhibitors of excessive release of mature, active TNFα. Also disclosed are methods for the identification of inhibitors of the conversion of the pro-form of TNFα to its mature active form.
Claims29
Isolated, purified recombinant human preproPR-3 comprising SEQ ID NO:
- 23.
A recombinant protein preparation comprising about 90% to about 99% by weight the isolated, purified recombinant human preproPR-3 comprising SEQ ID NO:
- 23.
Isolated, purified recombinant human proPR-3 comprising SEQ ID NO:
- 25.
A recombinant protein preparation comprising about 90% to about 99% by weight the isolated, purified recombinant human proPR-3 comprising SEQ ID NO:
- 25.
Isolated, purified recombinant human mature PR-3 comprising SEQ ID NO:
- 27.
A recombinant protein preparation comprising about 90% to about 99% by weight the isolated, purified recombinant human mature PR-3 comprising SEQ ID NO:
- 27.
An isolated, purified recombinant human preproPR-3 made by a process comprising:
- (a) transfecting an insect cell with a baculovirus vector containing a polynucleotide sequence encoding a human preproPR-3 protein comprising SEQ ID NO:
- 23;
- (b) expressing said preproPR-3 protein;
- and
- (c) isolating said preproPR-3 protein therefrom.
Dependencies are inferred from the claim text.