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AssigneeGenencor International, Inc.
InventorsThomas P. Graycar, Richard R. Bott, Lori J. Wilson
Estimating the term…
Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc. The substituted amino acid residues correspond to positions +76 in combination with one or more of the following residues +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 and/or +274 in Bacillus amyloliquefaciens subtilisin.
An isolated subtilisin variant having an amino acid sequence comprising a plurality of substitutions with a different naturally-occurring amino acid, said substitutions made at:
An isolated subtilisin variant having an amino acid sequence comprising a plurality of substitutions with a different naturally-occurring amino acid, said substitutions made at:
An isolated subtilisin variant having an amino acid sequence comprising combined substitution sets substituted with a different naturally-occurring amino acid, said combined substitution sets made at positions selected from the group consisting of the set of positions equivalent to 76/103/104/105, 76/103/104/210, 76/103/104/156/166/210, 76/101/103/104/166/210, and 76/101/103/104/156/166/210 in a Bacillus amyloliquefaciens subtilisin having an amino acid sequence set forth in SEQ ID No:7.
Dependencies are inferred from the claim text.