Method for cloning and expression of BsaWI restriction endonuclease and BsaWI methylase in E. coli
AssigneeNew England Biolabs, Inc.
InventorsShuang-yong Xu, Jing Zhou, Adam Hume, Robert Maunus
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Abstract
The present invention relates to recombinant DNA encoding the BsaWI restriction endonuclease as well as BsaWI methylase, expression of BsaWI restriction endonuclease and BsaWI methylase in E. coli cells containing the recombinant DNA.
Claims6
Isolated DNA encoding the BsaWI restriction endonuclease, wherein the isolated DNA is obtainable from Bacillus stearothermophilus W1718.
A recombinant DNA vector comprising a vector into which a DNA segment encoding the BsaWI restriction endonuclease has been inserted.
Isolated DNA encoding the BsaWI restriction endonuclease and BsaWI methylase, wherein the isolated DNA is obtainable from ATCC No. FTA-4100.
Dependencies are inferred from the claim text.