Polynucleotides encoding T1 receptor like ligand II
AssigneeHuman Genome Sciences, Inc.
InventorsJian Ni, Reiner L. Gentz, Steven M. Ruben
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Abstract
The present invention concerns a novel T1R-like ligand II protein. In particular, isolated nucleic acid molecules are provided encoding the T1R-like ligand II protein. T1R-like ligand II polypeptides are also provided, as are recombinant vectors and host cells for expressing the same.
Claims62
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding amino acids −26 to 203 of SEQ ID NO:2;
- (b) a nucleotide sequence encoding amino acids −25 to 203 of SEQ ID NO:2;
- (c) a nucleotide sequence encoding amino acids 1 to 203 of SEQ ID NO:2;
- (d) a nucleotide sequence encoding the complete polypeptide as encoded by the cDNA clone contained in ATCC Deposit No. 97655;
- (e) a nucleotide sequence encoding the mature polypeptide as encoded by the cDNA clone contained in ATCC Deposit No. 97655;
- and
- (f) the complement of (a), (b), (c), (d), or (e).
An isolated polynucleotide comprising 250 contiguous nucleotides of the coding region of SEQ ID NO:1, provided that said isolated polynucleotide does not have the nucleotide sequence of any one of SEQ ID NOs:12-25.
An isolated polynucleotide comprising a first polynucleotide which hybridizes at 42° C. in 50% formamide, 5×SSC, 50 mM sodium phosphate, 5×Denhardt's solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1×SSC at 65° C., to a second polynucleotide having the nucleotide sequence of the coding region of SEQ ID NO:1, or the complement thereof, wherein said first polynucleotide is 250 or more nucleotides long;
- and wherein said first polynucleotide does not have the nucleotide sequence of any one of SEQ ID NOs:12-25.
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of
- (a) a nucleotide sequence encoding amino acids 1 to 168 of SEQ ID NO:2;
- (b) a nucleotide sequence encoding amino acids 192 to 203 of SEQ ID NO:2;
- and
- (c) a nucleotide sequence encoding the extracellular and intracellular domains of the T1 receptor-like ligand II with all or part of the transmembrane domain deleted.
An isolated polynucleotide comprising a first nucleotide sequence 95% or more identical to a second nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding amino acids −26 to 203 of SEQ ID NO:2;
- (b) a nucleotide sequence encoding amino acids −25 to 203 of SEQ ID NO:2;
- (c) a nucleotide sequence encoding amino acids 1 to 203 of SEQ ID NO:2;
- (d) a nucleotide sequence encoding the complete polypeptide as encoded by the cDNA clone contained in ATCC Deposit No. 97655;
- (e) a nucleotide sequence encoding the mature polypeptide as encoded by the cDNA clone contained in ATCC Deposit No. 97655;
- and
- (f) the complement of (a), (b), (c), (d), or (e);
- wherein percent identity is calculated using BESTFIT with the parameters set such that percentage of identity is calculated over the full length of the reference nucleotide sequence and that gaps in homology of up to 5% of the total number of nucleotides in the reference sequence are allowed.
Dependencies are inferred from the claim text.