Xylanase production
AssigneeQuest International B.V.
InventorsRobert F. M. van Gorcom, Johanna G. M. Hessing, Jan Maat, Martinus Roza, Johannes Maria A. Verbakel
Life of this patent
Estimating the term…
Abstract
An isolated DNA with a nucleotide sequence encoding a ripening form of a xylanase fungal origin having bread improving activity. Cells are transformed with this DNA and used to produce the ripening form of xylanase which itself is suitable for use in flour and dough.
Claims41
A purified recombinant DNA material comprising a nucleotide sequence encoding a ripening from of a β-1,4 endoxylanase of fungal origin having bread improving activity, said nucleotide sequence being selected from the group consisting of the nucleotide sequence of SEQ ID NO:7, a nucleotide sequence encoding the amino acid sequence encoded by SEQ ID NO:7, a nucleotide sequence which hybridizes to the nucleotide sequence of SEQ ID NO:7, said hybridization being performed in 6×SSC at 68° C. followed by wash steps in 2×SSc and 0.4×SSC, respectively at 68° C., a nucleotide sequence containing SEQ ID NO:7 which encodes a mature form of β-1,4 endoxylanase, a nucleotide sequence containing SEQ ID NO:7 which encodes a preform of β-1,4 endoxylanase, a nucleotide sequence containing SEQ ID NO:7 which encodes a proform of β-1,4 endoxylanase and a nucleotide sequence containing SEQ ID NO:7 which encodes a preproform of β-1,4 endoxylanse.
A recombinant DNA material comprising a nucleotide sequence encoding a ripening form of a polypeptide having β-1,4 endoxylanase activity wherein said nucleotide sequence is selected from the group consisting of SEQ ID NO:7 and a nucleotide sequence encoding the polypeptide encoded by SEQ ID NO:7.
An isolated polypeptide comprising SEQ ID NO:8.
Dependencies are inferred from the claim text.