Isoprenoid production
AssigneeRoche Vitamins Inc.
InventorsTatsuo Hoshino, Kazuyuki Ojima, Yutaka Setoguchi
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Abstract
The present invention relates to an isolated DNA sequence encoding an enzyme in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate. Vectors and plasmids including such DNA are also set forth. The invention also includes host cells transformed by such DNAs, or vectors or plasmids containing such DNAs. A process for the production of isoprenoids and carotenoids using such transformed host cells is also provided.
Claims14
An isolated DNA sequence comprising a DNA sequence that hybridizes to SEQ ID NO:1 under the following conditions:
- hybridization in 50% formamide (v/v), 2% blocking agent, 5XSSC, 0.1% N-lauroylsarcosine (w/v), and 0.1% SDS at 42° C. overnight followed by two washes for 5 minutes each in 2XSSC and 0.1% SDS at room temperature followed by two additional washes of 15 minutes each in 0.1XSSC and 0.1% SDS at 68° C., wherein the DNA sequence encodes an amino acid sequence having 3-hydroxy-3-methylglutaryl-CoA synthase (HMG-CoA synthase) activity.
An isolated DNA sequence comprising a DNA sequence that does not hybridize to SEQ ID NO:1 but which encodes an amino acid sequence that is identical to SEQ ID NO:6.
Dependencies are inferred from the claim text.