Method of chromatographic isolation for non-glyceride components
OwnerChoo Yuen May, Ma Ah Ngan +1inventors
InventorsChoo Yuen May, Ma Ah Ngan, Yusof Basiron
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Abstract
A method of chromatographic isolation for non-glyceride components (squalenes, carotenes, vitamin E, sterols and/or the like) from a non-glyceride components-comprising compound by the steps of a. introducing the non-glyceride components-comprising compound onto a selective adsorbent to allow an adsorption of the non-glyceride components, and subsequently b. desorbing the non-glyceride components from the adsorbent, wherein the adsorption and/or desorption of the non-glyceride components is carried out under a supercritical fluid environment.
Claims41
A method for the chromatographic isolation of squalenes, sterols, vitamin E, and carotenes from a vegetable oil comprising free fatty acid components and glyceride components comprising:
- a. esterifying the free fatty acid components with one or more monohydric alcohols to provide an esterified composition having a fatty acid content that is less than 0.5% and transesterifying the glyceride components of the esterified composition into monoesters to form an esterified and transesterified composition;
- b. separating the squalenes, sterols, vitamin E, and carotenes from the esterified and transesterified composition by adsorbing the squalenes, sterols, vitamin E, and carotenes on a single type of adsorbent and then desorbing the squalenes, sterols, vitamin E, and carotenes from the adsorbent,
- wherein the squalenes, sterols, vitamin E, and carotenes are adsorbed and desorbed in a single run of separation, using a supercritical fluid, under isochratic conditions, at a constant temperature, and a constant flow rate.
A method for the chromatographic isolation of squalenes, sterols, vitamin E, and carotenes from a vegetable oil comprising free fatty acid components and glyceride components comprising:
- a. esterifying the free fatty acid components with one or more monohydric alcohols to provide an esterified composition having a fatty acid content of less than 0.5% and transesterifying the glyceride components of the esterified composition with one or more monohydric alcohols to provide monoesters to form an esterified and transesterified composition;
- b. removing at least 90% of the esterified fatty acids and monoesters from the esterified and transesterified composition by vacuum distillation to provide a phytonutrient concentrate;
- c. separating the squalenes, sterols, vitamin E, and carotenes from the phytonutrient concentrate by adsorbing the squalenes, sterols, vitamin E, and carotenes on an adsorbent and then desorbing the squalenes, sterols, vitamin E, and carotenes from the adsorbent,
- wherein the squalenes, sterols, vitamin E, and carotenes are adsorbed and desorbed under a supercritical fluid environment.
A method for the chromatographic isolation of squalenes, sterols, vitamin E, and carotenes from a vegetable oil comprising free fatty acid components and glyceride components comprising:
- a. esterifying the free fatty acid components with one or more monohydric alcohols to provide an esterified composition having a fatty acid content that is less than 0.5% and transesterifying the glyceride components of the esterified composition with one or more monohydric alcohols to provide monoesters to form an esterified and transesterified composition;
- b. removing at least 90% of the esterified fatty acids and monoesters from the esterified and transesterified composition by vacuum distillation to provide a phytonutrient concentrate;
- c. separating the squalenes, sterols, vitamin E, and carotenes from the phytonutrient concentrate by adsorbing the squalenes, sterols, vitamin E, and carotenes on a single type of adsorbent and then desorbing the squalenes, sterols, vitamin E, and carotenes from the adsorbent;
- wherein the squalenes, sterols, vitamin E, and carotenes are adsorbed and desorbed in a single run of separation, using a supercritical fluid, under isochratic conditions, at a constant temperature, and a constant flow rate.
Dependencies are inferred from the claim text.