Loading patent…
AssigneeThe Kitasato Institute
InventorsSatoshi Omura, Akio Abe
Estimating the term…
The present invention relates to a method for detecting substances specifically inhibiting a type III secretion mechanism and functions of the type III secretory proteins, within short time and large amounts thereof, without depending upon animal infectious experiments. Namely it relates to the method for detection of a type III secretory mechanism inhibitor comprising mixing a bacterium having the type III secretory mechanism and an erythrocyte suspension, adding the type III secretory mechanism inhibitor thereto, and detecting changes in the thus formed hemolytic activity. The method for detecting substances can be treated large amount of samples within short time by exhibiting the substances inhibiting the type III secretion mechanism or the functions of the type III secretory proteins as numerical index of the hemolytic activity of erythrocytes. Consequently, the present invention is useful for development of drugs.
A method for detection of a type III secretory mechanism inhibitor comprising mixing a bacterium having the type III secretory mechanism and an erythrocyte suspension, adding the type III secretory mechanism inhibitor thereto, and detecting changes in the thus formed hemolytic activity which indiates the effectiveness of a type III secretory mechanism inhibitor.
A method for detection of a substance inhibiting function of secretory protein of type III secretory mechanism comprising mixing a bacterium having the type III secretory mechanism and an erythrocyte suspension, adding the substance inhibiting the function of protein secreted by the type III secretory mechanism thereto, and detecting changes in the thus formed hemolytic activity which indicates the effectiveness of a substance inhibiting the function of type III secretory mechanism.
Dependencies are inferred from the claim text.