Methods and materials for identifying novel pesticide agents
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsMichael J. Adang, Ke Luo
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Abstract
Materials and methods for identifying novel pesticide agents are disclosed herein. Specifically exemplified is a full length aminopeptidase N isolated from Manduca sexta, insect cells expressing APN, and methods of screening pesticide agents using the same. Also disclosed are methods of identifying novel APN inhibitors.
Claims9
An isolated polynucleotide comprising the sequence depicted in SEQ ID NO:
- 2, or a fragment thereof sufficient to encode a functional protein.
A transgenic cell comprising a heterologous polyaucleotide encoding an aminopeptidase N (APN), wherein said polynucleotide comprises the sequence depicted in SEQ ID NO:
- 2 or a fragment thereof sufficient to encode a fictional protein.
A method of identifying a cytotoxic agent wherein said method comprises the steps of obtaining a transgenic cell comprising a heterologous polynucleotide encoding an APN, wherein said polynucleotide comprises the sequence depicted in SEQ ID NO:
- 2 or a fragment thereof sufficient to encode a functional protein;
- and using said cell to screen at least one compound for its ability to produce a cytotoxic effect on said cell, whereby a cytotoxic agent is identified.
A method of screening for aminopeptidase inhibitors wherein said method comprises the steps of:
- a) obtaining a plurality of transgenic cells comprising a heterologous polynucleotide encoding an APN, wherein said polynucleotide comprises the sequence depicted in SEQ D NO:
- 2 or a fragment thereof sufficient to encode a functional protein;
- b) exposing said cells to at least one compound of interest;
- and
- c) monitoring said exposed cells for an inhibitive effect attributable to said exposure step.
Dependencies are inferred from the claim text.