Multiple enzyme assays

A kit for detecting the activity of multiple enzymes in a single aliquot of a sample comprising:

• reagents for quantifying each enzyme;
• substrates for each of said enzymes;
• and
• an accelerator solution containing a water soluble polymeric enhancer molecule;
• wherein at least one of said multiple enzymes is an endogenous enzyme.

An assay for measuring the activity of multiple enzymes in a single aliquot of a sample comprising:

• (a) quantifying the activity of an enzyme by measuring the light signal produced by the degradation of an enzyme substrate specific for said enzyme by said enzyme;
• (b) repeating step (a) for each enzyme present in said aliquot that is to be measured;
• wherein said enzymes are selected from the group consisting of reporter enzymes and endogenous enzymes;
• wherein at least one of said enzymes is an endogenous enzyme;
• and
• wherein the activity for each enzyme measured is quantified simultaneously.

An assay for simultaneously measuring the activity of multiple enzymes in a single aliquot of a sample comprising:

• simultaneously quantifying the activity of enzymes by simultaneously measuring light signals produced by the degradation of enzyme substrates specific for each of said enzymes by said enzymes;
• wherein said enzymes are selected from the group consisting of reporter enzymes and endogenous enzymes;
• wherein at least one of said enzymes is an endogenous enzyme;
• wherein said light signals are distinguishable from each other;
• and
• wherein one or more enhancers are added to enhance said light signals.