Method of detecting sugars
AssigneeR.E. Davis Chemical Corporation
InventorArthur J. Friedman
Life of this patent
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Abstract
A method for detecting reducing sugars in samples using a test reagent containing an indicator capable of producing calorimetric changes when contacted with reducing sugars. Under alkaline conditions, reducing sugars present in a sample affect a reduction of the indicator to a colorless or differently colored reduced form. The method may include the additional step of pretreating the sample with a glycosidase to convert non-reducing sugars to reducing sugars.
Claims10
A method of detecting reducing sugars in a sample comprising the steps of:
- contacting a test reagent with the sample, the test reagent having an alkaline pH and including an indicator capable of providing a colorimetric change when contacted with reducing sugar in the sample;
- subsequently detecting a colorimetric change in the indicator indicating the presence of reducing sugars in the sample.
A method of detecting reducing sugars in a sample comprising the steps of
- contacting a test reagent with the sample, the test reagent having an alkaline pH and including a phenazine compound capable of providing a calorimetric change when contacted with reducing sugar in the sample;
- subsequently detecting a calorimetric change in the phenazine compound indicating the presence of reducing sugars in the sample.
A method of detecting reducing sugars in a sample comprising the steps of:
- contacting a test reagent with the sample, the test reagent having an alkaline pH and including an indicator selected from the group consisting of azocarmine B, indoine blue, methylene violet 3 RAX, safranine O, phenosafranine, and Janus Green B, the indicator capable of providing a calorimetric change when contacted with reducing sugar in the sample;
- subsequently detecting a colorimetric change in the indicator indicating the presence of reducing sugars in the sample.
A method of detecting sugars comprising the steps of:
- pretreating the sample with a glycosidase to form a pretreated sample;
- contacting a test reagent with the pretreated sample, the test reagent having an alkaline pH and including a phenazine compound capable of providing a calorimetric change when contracted with reducing sugar within the sample;
- subsequently detecting a colorimetric change in the indicator indicating the presence of reducing sugars in the sample.
Dependencies are inferred from the claim text.