Analyte assay using particulate labels
AssigneesGenicon Sciences Corporation, The Regents of the University of California
InventorsJuan Yguerabide, Evangelina E. Yguerabide, David E. Kohne, Jeffrey T. Jackson
Life of this patent
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Abstract
Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.
Claims73
A method for specific detection of one or more analytes in a sample, comprising the steps of:
- (a) providing a plurality of distinct populations of scattered-light detectable particles of a size between 1 and 500 nm inclusive, wherein each population specifically binds to a different predetermined analyte, and has a particle type configuration distinguishable from other populations by its predetermined scattered-light detectable property;
- (b) contacting the sample with said particles;
- (c) illuminating said particles using non-evanescent wave light under conditions which produce scattered light from said particle and in which light scattered from one or more said particles can be detected by a human eye with less than 500 times magnification and without electronic amplification;
- and
- (d) detecting light scattered by (i) said populations of particles bound with analyte under said conditions;
- or (ii) said populations of particles not bound with analyte under said conditions;
- or (iii) both (i) and (ii), as a measure of the presence of said bound analyte.
A method for specific detection of one or more analytes in a sample, comprising the steps of:
- (a) providing a plurality of distinct populations of scattered-light detectable particles of a size between 1 and 500 nm inclusive, wherein each population specifically binds to a different predetermined analyte, and has a particle type configuration distinguishable from other populations by its predetermined scattered-light detectable property;
- (b) contacting the sample with said particles;
- (c) illuminating any said particles with non-evanescent wave light under conditions which produce scattered light from said particle and in which light scattered from one or more said particles can be detected by a human eye with less than 500 times magnification and without electronic amplification;
- and
- (d) detecting light scattered by (i) said populations of particles bound with analyte under said conditions;
- or (ii) said populations of particles not bound with analyte under said conditions;
- or (iii) both (i) and (ii), as a measure of the presence of said bound analytes,
- wherein said detecting comprises flowing said particles by at least one detector.
Dependencies are inferred from the claim text.