Parallel high throughput method and kit

A kit for screening a plurality of target proteins from a genome comprising:

• a) sets of target modules, wherein each set of said sets of target modules comprises:
• (1) individually detectable beads;
• (2) a set of surrogate ligands attached to said detectable beads, wherein the surrogate ligands of said set of surrogate ligands are peptides and are bound selectively to the same or different target protein of said plurality of target proteins;
• (3) wherein said target proteins are labeled for detection;
• and
• b) a multi-chamber container, wherein said sets of target modules are stored in each chamber of said mult-chamber container.

A kit for screening a plurality of target proteins from a genome comprising:

• a) a set of ligand-bead complexes, wherein each said ligand-bead complex comprises:
• (1) a detectable bead;
• (2) a surrogate ligand comprising a peptide attached to said detectable bead;
• b) a plurality of target proteins, each said target protein of said plurality of target proteins is capable of selectively binding to a surrogate ligand;
• and
• c) a multi-chamber container, wherein said plurality of ligand-bead complexes are stored in each chamber of said multi-chamber container, said target proteins being stored separately from said ligand-bead complexes.

In a method for high throughput screening using individually detectable beads, surrogate ligands, and a plurality of target proteins, the improvement comprising,

• a) combining in each chamber of a multi-chamber container sets of target modules,
• i) wherein each target module within each set of said sets of target modules comprises a ligand-bead complex and a target protein labeled for detection;
• ii) wherein said ligand-bead complex comprises a surrogate ligand comprising a peptide coupled to an individually detectable bead;
• iii) wherein each said surrogate ligand binds selectively to one of said target proteins;
• b) adding to each said chamber of said multi-chamber container a test compound, whereby said compound displaces said target proteins from said target modules to which said target compounds interact;
• c) detecting displacement of a target protein with a test compound;
• and
• d) determining the identity of each target protein that is displaced with a test compound.