US 6,586,180 B1 · Directed antisense libraries

Abstract

A method for making a directed antisense library against a target transcript is described. A cDNA of the target transcript is cloned in an appropriate cloning vector. Next, a plurality of deletion derivatives of the cloned cDNA is prepared such that the deletions serially extend into the cDNA from one end thereof. The resulting deletion library is then treated such that cDNA is removed from the other end of each cDNA insert, thus obtaining a fragment library having fragments of a selected size. A catalytic core is then inserted into each fragment of the fragment library, resulting in the directed antisense library. An illustrative antisense gene in the hammerhead ribozyme catalytic core. Plasmids for making the antisense library, plasmids and methods for making the fragment library, and a method for identifying target sites for antisense-mediated gene inhibition are also described.

Claims8

Dependent claims

A method for generating an oligonucleotide library targeted to a selected RNA transcript comprising:

(a) preparing a double stranded cDNA, comprising a first end, a second end, and a central site thereof, from the selected RNA transcript and cloning the cDNA in a cloning vector comprising a promoter configured such that an antisense transcript of the cDNA is synthesized upon transcription mediated by the promoter, resulting in a cloned cDNA;
(b) creating a plurality of deletion derivatives of said cloned cDNA by exonuclease resection thereof, wherein each of said plurality of deletion derivatives has a deletion extending from said first end towards the central site of the cloned cDNA such that the plurality of deletion derivatives comprises a deletion library comprising deletions that extend serially into the cDNA;
and
(c) reducing the size of the cDNA contained in the deletion library to a preselected size by removing a portion of the cDNA from the second end thereof to result in a fragment library;
(d) inserting a catalytic core into the central site of the cDNA in the fragment library, thereby obtaining the oligonucleotide library.

2 more elements

Dependencies are inferred from the claim text.

Directed antisense libraries

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Directed antisense libraries

Life of this patent

Abstract

Claims8