Human Eag2
AssigneeIcagen, Inc.
InventorsTimothy J. Jegla, Yi Liu
Life of this patent
Estimating the term…
Abstract
The invention provides isolated nucleic acid and amino acid sequences of Eag2, antibodies to Eag2, methods of detecting Eag2, and methods of screening for modulators of Eag2 potassium channels using biologically active Eag2. The invention further provides, in a computer system, a method of screening for mutations of human Eag2 genes as well as a method for identifying a three-dimensional structure of Eag2 polypeptide monomers.
Claims12
An isolated nucleic acid encoding a polypeptide comprising an alpha subunit of a potassium channel, wherein the subunit:
- (i) forms, with at least one additional Eag family alpha subunit, a potassium channel having the characteristic of voltage sensitivity;
- and wherein said nucleic acid specifically hybridizes under stringent conditions to SEQ ID NO:1, wherein the hybridization reaction is incubated at 42° C. in a solution comprising 50% formamide, 5×SSC, and 1% SDS or at 65° C. in a solution comprising 5×SSC and 1% SDS, with a wash in 0.2×SSC and 0.1% SDS at 65° C.
An isolated nucleic acid encoding a polypeptide comprising an alpha subunit of a potassium channel, wherein the subunit:
- (i) forms, with at least one additional Eag family alpha subunit, a potassium channel having the characteristic of voltage sensitivity;
- and
- (ii) comprises an amino acid sequence that has greater than 85% amino acid identity to the amino acid sequence of SEQ ID NO:2.
Dependencies are inferred from the claim text.