Nucleic acid probe
AssigneeTosoh Corporation
InventorsRyuichi Horie, Takahiko Ishiguro, Takumi Tokunaga, Takashi Yamamoto, Juichi Saitoh, Toshitaka Taya
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Abstract
A nucleic acid probe which is a single-stranded nucleic acid complementary to a specific nucleic acid sequence and is labeled so as to give off a measurable fluorescent signal on hybridization with a nucleic acid containing the specific nucleic acid sequence, wherein the 3′ end of the probe is modified as represented by the following formula (1): wherein R is —COOH, —CONH2, —(CH2)nOH, —CH(OH)—CH2OH, or —CH[(CH2)n—NHR1]—CH2OH, R1 is H, a dye, or an amino protecting group such as Fmoc, and n is an integer of at least 1.
Claims12
A nucleic acid probe which is a single-stranded nucleic acid complementary to a specific nucleic acid sequence and is labeled so as to give off a measurable fluorescent signal on hybridization with a nucleic acid containing the specific nucleic acid sequence, wherein the 3′ end of the probe is modified as represented by the following formula (1):
- wherein R is —COOH, —CONH2, —(CH2)nOH, —CH(OH)—CH2OH, or —CH[(CH2)n—NHR1]—CH2OH, R1 is H, a dye, or an amino protecting group, and n is an integer of at least 1,
- wherein the single-stranded nucleic acid is DNA having an oxygen atom retained at position 3 of the deoxyribose moiety.
A method for assay of a target nucleic acid containing a specific base sequence, which comprises amplifying the target nucleic acid in the presence of a single-stranded nucleic acid probe complementary to the specific nucleic acid sequence which is labeled so as to give off a measurable signal on hybridization with a nucleic acid containing the specific nucleic acid sequence and detecting or quantifying the specific nucleic acid by using hybridization of the amplified target nucleic acid with the nucleic acid probe during and/or after the amplification, wherein the 3′ end of the single-stranded nucleic acid probe is modified as represented by the following formula (2):
- wherein R is —COOH, —CONH2, —(CH2)nOH, —CH(OH)—CH2OH, or —CH[(CH2)n—NHR1]—CH2OH, R1 is H, a dye, or an, and n is an integer of at least 1,
- wherein the single-stranded nucleic acid is DNA having an oxygen atom retained at position 3 of the deoxyribose moiety.
Dependencies are inferred from the claim text.