US 6,586,174 B1 · Suppressor of HIV-1 replication and transcription

Abstract

The present invention relates to a bioactive molecule, herein referred to as the CD8+ suppressor molecule, that is produced by the CD8+ subset of human T-lymphocytes and suppresses type-1 human immunodeficiency virus (HIV-1) replication through inhibition of viral transcription. The invention relates to isolation of clonal CD8+ cells lines that produce the antiviral activity and the development of an assay system for detection of the antiviral activity. The clonal cell lines and the assay system, described herein, may be utilized to purify, characterize and clone the CD8+ suppressor molecule. The CD8+ suppressor molecule may have therapeutic applications for treatment of diseases associated with HIV-1 infection.

Claims1

Dependent claims

A method for detecting a CD8+ suppressor molecule that has anti-HIV activity, comprising:

(a) contacting a culture of cells with a sample containing a suppressor molecule,
(i) wherein the culture of cells comprises cells genetically engineered to contain recombinant DNA expression vector in which a reporter gene is operatively associated with an HIV LTR promoter sequence, and
(ii) wherein the sample containing the CD8+ suppressor molecule comprises culture supernatant from CD8+ cells or conditioned medium from CD8+ cells;
and
(b) measuring the inhibition of reporter gene activity, wherein inhibition of reporter gene activity correlates with anti-HIV activity.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

09/450,141Filed 29 Nov 1999 · division

Priority

29 Mar 1993

Granted

1 Jul 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12Q 1/703
For retroviruses: viruses associated with AIDS
C07K 14/4703
Regulators; Modulating activity: inhibitors; Suppressors
C12Q 1/6897
Involving nucleic acids: involving reporter genes operably linked to promoters
A61K 38/00
Medicinal preparations containing peptides

Family

Application08/038,387Parent of this continuation-in-part · filed 29 Mar 1993

Application08/471,430Parent of this division · filed 6 Jun 1995

Application08/486,810Parent of this division · filed 7 Jun 1995

US 5,994,054 AParent of this division

US 5,861,490 AParent of this division

Application08/471,430Parent of this continuation-in-part

US 5,627,023 AParent of this continuation-in-part

US 6,586,174 B1This patent

From the US filing history. Foreign family members aren’t listed.

Suppressor of HIV-1 replication and transcription

Life of this patent

Suppressor of HIV-1 replication and transcription

Life of this patent

Abstract

Claims1