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US 6,586,021 B2Granted
Method for extraction and purification of biologically useful molecules from a mangrove plant Salvadora persica L
AssigneeCouncil of Scientific and Industrial Research
InventorsUsha Goswami, Nazarine Fernandes
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Abstract
The invention discloses a process of extracting, fractionating and purifying bioactive molecules from an associated mangrove plant, methods of screening for pharmacological activities of the crude extract, fractions, and purified compounds.
Claims1
A process for extraction and purification of biologically useful molecules β-amyrin, ursolic acid, methyl palmitate, and lupeol from mangrove plant Salvadora persica comprising the steps of:
- (a) drying said plant;
- (b) cutting said dried plant into small pieces;
- (c) immersing said small plant pieces into about 85-95% aqueous methanol for about 5-15 days to obtain an extract;
- (d) evaporating said aqueous methanol from said obtained extract under reduced pressure at a temperature of from 45° to 55° C.;
- (e) cold-percolating said extract at room temperature;
- (f) repeating steps (c)-(e) at least twice to obtain a concentrated crude extract;
- (g) fractionating said concentrated crude extract into a petroleum ether fraction, a chloroform fraction, an n-butanol fraction, and an aqueous fraction using separate funnels;
- (h) identifying said aqueous fraction as the fraction containing said biologically useful molecules;
- (i) isolating said biologically useful molecules via chromatography;
- and
- (j) identifying said compounds via spectroscopy.
8 more elements
Dependencies are inferred from the claim text.