Methods to facilitate the calculation of yields of reaction products

A method for calculating the yields of the products of a reaction using a mass spectrometer, the method comprising:

• counting the number of ions of reference materials and reaction products that have been cleaved from multiple solid supports;
• determining the efficiency with which the reference materials and the products may be detected by a given mass spectrometer, wherein the efficiency relates at least in part to the efficiency with which the reference materials and the products are transferred from droplet phase to gas phase or are transferred to a low pressure side of the mass spectrometer;
• and
• determining the percentage yield of each reaction product based at least in part on the amount of the number of counted ions of the associated reference material, the counted number of ions for the reaction product, and the determined efficiency.

A method for evaluating the yields of multiple chemical reactions, the method comprising:

• providing multiple solid supports which each include with a first link, a reference material, and a second link having an attachment site;
• performing chemistry on the attachment sites of the second links in one or more steps and under different reaction conditions to produce a reaction product for each solid support;
• and
• determining the percentage yield of the reaction products based at least in part the amount of measured reference material;
• and
• associating the yields with the reaction conditions.

A method for calculating the yield of a reaction product, the method comprising:

• providing a construct with a first link, a reference material, and a second link having an attachment site;
• performing chemistry on the attachment site of the second link in one or more steps to produce a reaction product;
• measuring the amount of the reference material and the reaction product;
• determining the percentage yield of the reaction product based at least in part on the amount of measured reference material and the amount of measured reaction product.