Stimulation of homologous recombination in eukaryotic organisms or cells by recombination promoting enzymes
AssigneeBASF Plant Science GmbH
InventorsBernd Reiss, Hans Kosak, Manfred Klemm, Jeff Schell
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Abstract
The present invention relates to a novel process for the production of transgenic organisms or transgenic cells, to transgenic orgaisms or transgenic cells obtainable by the process of the present invention, to the use of vectors comprising DNA encoding a recombination promoting enzymes for curing impairments caused by environmental influences in plants or plant cells and for gene therapy in mammals or mammalian cells, and to novel vectors.
Claims20
A method for the production of a transgenic plant or a transgenic plant cell with enhanced recombination, comprising
- (1) insertion of at least a first and second DNA sequence into the genome of said plant or plant cell, said first DNA comprising
- (a) the sequence of at least one gene of interest, and
- (b) a sequence which encodes a selection marker expressible in said plant or plant cell, and
- said second DNA comprising a sequence that encodes a RecA operatively linked to a DNA sequence encoding a nuclear targeting sequence for promoting homologous recombination, and
- (2) selection of transgenic plants or plant cells which have taken up said DNA, and
- (3) culturing of said transgenic plant or transgenic plant cells in a suitable culture medium.
A method for the production of a transgenic plant or a transgenic plant cell with enhanced recombination, comprising
- (1) insertion of DNA into the genome of a plant or plant cell, said DNA comprising
- (a) the sequence of at least one gene of interest, and
- (b) a sequence which encodes a selection marker expressible in said plant or plant cell, and
- (c) a nucleic acid molecule encoding the amino acid sequence of the E. coli RecA protein,
- wherein said nucleic acid molecule is operatively linked to a DNA sequence encoding a nuclear targeting sequence,
- (2) selection of desired transgenic plants or transgenic plant cells which have taken up said DNA, and
- (3) culturing of said desired transgenic plants or transgenic plant cells in a suitable culture medium.
A vector which comprises a DNA sequence encoding a nuclear targeting sequence operatively linked to a sequence that encodes a RecA and further comprising a DNA sequence encoding a selectable marker.
A vector which comprises a DNA sequence encoding a T SV40 nuclear targeting sequence operatively linked to a sequence that encodes a RecA and further comprising a DNA sequence encoding a selection marker and the DNA sequence of at least one gene of interest.
A method for the production of a transgenic plant or a transgenic plant cell with enhanced recombination, comprising
- (1) insertion of DNA into the genome of a plant or plant cell, said DNA comprising
- (a) the sequence of at least one gene of interest, and
- (b) a sequence which encodes a selection marker expressible in said plant or plant cell, and
- (c) a nucleic acid molecule encoding the amino acid sequence of the E. coli RecA protein,
- and wherein said nucleic acid molecule of (c) is operatively linked to a DNA sequence encoding the T SV40 nuclear targeting sequence,
- (2) selection of desired transgenic plants or transgenic plant cells which have taken up said DNA, and
- (3) culturing of said desired transgenic plants or transgenic cells in a suitable culture medium.
A vector which comprises a DNA sequence encoding a T SV40 nuclear targeting sequence operatively linked to a
- a nucleic acid sequence encoding the amino acid sequence of the E. coli RecA protein,
- wherein said vector further comprises a promoter that functions in plants, said promoter operably linked to said DNA sequence.
A vector which comprises a DNA sequence encoding a T SV40 nuclear targeting sequence operatively linked to a sequence that encodes RecA that mediates strand-exchange and further comprising a DNA sequence encoding a selection marker and the DNA sequence of at least one gene of interest.
Dependencies are inferred from the claim text.