Ovariectomized mouse model for human menopause
AssigneeBoard of Trustees operating Michigan State University
InventorSandra Z. Haslam
Life of this patent
Estimating the term…
Abstract
A method for determining a response to a biologically active agent in ovariectomized lower test mammals is described. The mammals are divided into those which have an early time post ovariectomy and those which have a late time post ovariectomy and then each group is tested simultaneously with the agent. The method is used to simulate menopause in humans for the purpose of determining a positive or negative response and a possible treatment.
Claims23
A method for determining a response to a biologically active agent in ovariectomized mice which comprises:
- (a) surgically ovariectomizing the mice at a baseline time to establish menopause in the mice;
- (b) monitoring the ovariectomized mice for serum estrogen after being ovariectomized and establishing a constant level of estrogen for the overiectomized mice;
- (c) dividing the ovariectomized mice of step (b) into at least two groups;
- (d) treating a first of the groups of the ovariectomized mice over time with the biologically active agent wherein the treating is initiated within a first period of time which is less than 21 days from the baseline time and which corresponds to early menopause in a human;
- (e) treating a second of the groups of the ovariectomized mice over time with the biologically active agent wherein the treating is initiated within a second period of time which is greater than about 35 days from the baseline time and which corresponds to late menopause in a human;
- and
- (f) comparing the results for the first and second groups of ovariectomized mice to determine the response to the biologically active agent.
A method for determining the effect of a biologically active agent on an organ system in ovariectomized mice that undergo hormone replacement therapy (HRT) after ovariectomy which comprises:
- (a) surgically ovariectomizing the mice at a baseline time to establish menopause in the mice;
- (b) dividing the ovariectomized mice into at least two groups;
- (c) administering the HRT over time to a first of the groups of the ovariectomized mice wherein the HRT is initiated within a first period of time which is within about 21 days from the baseline time and which is the period of time that corresponds to early menopause in the human;
- (d) administering the HRT over time to a second of the groups of the ovariectomized mice wherein the HRT is initiated within a second period of time which is about 35 or more days from the baseline time and which is the period of time that corresponds to late menopause in the human;
- (e) treating the ovariectomized mice in the first and the second groups being administered the HRT with the biologically active agent;
- and
- (f) determining the effect of the biologically active agent on the organ system of the mice being administered the HRT.
A method for determining a response to a biologically active agent in mice that experience menopause when ovariectomized which comprises:
- (a) surgically ovariectomizing the mice at a baseline time to establish menopause in the mice;
- (b) monitoring the ovariectomized mice for serum estrogen after being ovariectomized and establishing a constant level of estrogen for the ovariectomized mice;
- (c) dividing the ovariectomized mice of step (b) into at least two groups;
- (d) treating a first of the groups of the ovariectomized mice over time with the biologically active agent wherein the treating is initiated within a first period of time after the baseline time which corresponds to early menopause in a human;
- (e) treating a second of the groups of the ovariectomized mice over time with the biologically active agent wherein the treating is initiated within a second period of time after the baseline time which corresponds to late menopause in a human;
- and
- (f) comparing the results for the first and second groups of ovariectomized mice to determine the response to the biologically active agent.
Dependencies are inferred from the claim text.