Nucleic acid sequences and expression system relating to Enterococcus faecium for diagnostics and therapeutics
AssigneeGenome Therapeutics Corporation
InventorsLynn A. Doucette-Stamm, David Bush
Life of this patent
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Abstract
The invention provides isolated polypeptide and nucleic acid sequences derived Enterococcus faecium that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.
Claims34
An isolated nucleic acid consisting of a nucleotide sequence encoding an E. faecium polypeptide selected from the group consisting of SEQ ID NO:
- 3857, SEQ ID NO:
- 4234, SEQ ID NO:
- 4304, SEQ ID NO:
- 4368, SEQ ID NO:
- 5256, SEQ ID NO:
- 5965, SEQ ID NO:
- 5985, SEQ ID NO:
- 6156, SEQ ID NO:
- 6320, and SEQ ID NO:
- 7224.
A single-stranded probe comprising a nucleotide sequence of at least 40 sequential nucleotides selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570.
An isolated nucleic acid or the complement thereof consisting of at least 30 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570, wherein the isolated nucleic acid is hybridizable under conditions of high stringency to a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570;
- wherein the complement indicates full complementarity and of the same length.
An isolated nucleic acid selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570.
An isolated nucleic acid consisting of a nucleotide sequence encoding an E. faecium polypeptide wherein said isolated nucleic acid consists of at least 40 sequential nucleotides selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570.
A single-stranded probe consisting of a nucleotide sequence consisting of at least 40 sequential nucleotides selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570.
An isolated nucleic acid or the complement thereof consisting of at least 20 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570, wherein the isolated nucleic acid is hybridizable under conditions of high stringency to a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570;
- wherein the complement indicates full complementarity and of the same length.
An isolated nucleic acid or the complement thereof consisting of at least 30 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570, wherein the isolated nucleic acid has at least 80% sequence identity with a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570 and complements thereof;
- wherein the complement or complements indicates full complementarity and of the same length.
An isolated nucleic acid or the complement thereof consisting of at least 30 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570, wherein the isolated nucleic acid has at least 70% sequence identity with a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, SEQ ID NO:
- 3570 and complements thereof wherein the complement or complements indicates full complementarity and of the same length.
An isolated nucleic acid or the complement thereof consisting of at least 20 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570, wherein the isolated nucleic acid has at least 90% sequence identity with a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, SEQ ID NO:
- 3570 and complements thereof;
- wherein the complement or complements indicates full complementarity and of the same length.
An isolated nucleic acid consisting of a nucleotide sequence with at least 70% sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570 and wherein said isolated nucleic acid encodes an E. faecium polypeptide.
An isolated nucleic acid consisting of a nucleotide sequence with at least 80% sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570 and wherein said isolated nucleic acid encodes an E. faecium polypeptide selected from the group consisting of SEQ ID NO:
- 3857, SEQ ID NO:
- 4234, SEQ ID NO:
- 4304, SEQ ID NO:
- 4368, SEQ ID NO:
- 5256, SEQ ID NO:
- 5965, SEQ ID NO:
- 5985, SEQ ID NO:
- 6156, SEQ ID NO:
- 6320, and SEQ ID NO:
- 7224, respectively.
An isolated nucleic acid consisting of a nucleotide sequence with at least 90% sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 203, SEQ ID NO:
- 580, SEQ ID NO:
- 650, SEQ ID NO:
- 714, SEQ ID NO:
- 1602, SEQ ID NO:
- 2311, SEQ ID NO:
- 2331, SEQ ID NO:
- 2502, SEQ ID NO:
- 2666, and SEQ ID NO:
- 3570 and wherein said isolated nucleic acid encodes an E. faecium polypeptide selected from the group consisting of SEQ ID NO:
- 3857, SEQ ID NO:
- 4234, SEQ ID NO:
- 4304, SEQ ID NO:
- 4368, SEQ ID NO:
- 5256, SEQ ID NO:
- 5965, SEQ ID NO:
- 5985, SEQ ID NO:
- 6156, SEQ ID NO:
- 6320, and SEQ ID NO:
- 7224, respectively.
A single-stranded probe comprising a nucleotide sequence of at least 30 sequential nucleotides selected from the group consisting of SEQ ID NO:
- 1602 and SEQ ID NO:
- 2311.
An isolated nucleic acid consisting of a nucleotide sequence encoding an E. faecium polypeptide wherein said isolated nucleic acid consists of at least 30 sequential nucleotides selected from the group consisting of SEQ ID NO:
- 1602 and SEQ ID NO:
- 2311.
An isolated nucleic acid consisting of a nucleic acid encoding an E. faecium polypeptide wherein said isolated nucleic acid consists of at least 20 sequential nucleotides selected from the group consisting of SEQ ID NO:
- 1602 and SEQ ID NO:
- 2311.
A single-stranded probe consisting of a nucleotide sequence consisting of at least 20 sequential nucleotides selected from the group consisting of SEQ ID NO:
- 1602 and SEQ ID NO:
- 2311.
Dependencies are inferred from the claim text.