System for the in vivo delivery and expression of heterologous genes in the bone marrow

A method of introducing and expressing heterologous RNA in bone marrow cells, comprising:

• (a) providing a recombinant South African Arbovirus No. 86 (S.A.AR86), said S.A.AR86 containing a heterologous RNA to be expressed in said bone marrow cells;
• and
• (b) contacting said recombinant S.A.AR86 to said bone marrow cells so that said heterologous RNA is introduced and expressed therein.

A method of introducing and expressing heterologous RNA in bone marrow cells, comprising:

• (a) providing a recombinant Girdwood S.A. virus, said Girdwood S.A. virus containing a heterologous RNA to be expressed in said bone marrow cells;
• and
• (b) contacting said recombinant Girdwood S.A. virus to said bone marrow cells so that said heterologous RNA is introduced and expressed therein.

A method of introducing and expressing heterologous RNA in bone marrow cells, comprising:

• (a) providing a recombinant Sindbis virus, said Sindbis virus containing a heterologous RNA to be expressed in said bone marrow cells;
• and
• (b) contacting said recombinant Sindbis virus to said bone marrow cells so that said heterologous RNA is introduced and expressed therein.

A method of introducing and expressing heterologous RNA in bone marrow cells, comprising:

• (a) providing a recombinant Sindbis strain TR339 virus, said Sindbis strain TR339 virus containing a heterologous RNA to be expressed in said bone marrow cells;
• and
• (b) contacting said recombinant Sindbis strain TR339 virus to said bone marrow cells so that said heterologous RNA is introduced and expressed therein.