Human bikunin
AssigneeBayer Aktiengesellschaft
InventorsPaul P. Tamburini, Gary Davis, Katherine A. Delaria, Christopher W. Marlor, Daniel K. Muller
Life of this patent
Estimating the term…
Abstract
The instant invention provides for proteins, polypeptides, nucleic acid sequences, constructs, expression vectors, host cells, pharmaceutical compositions of, and methods for using human placental bikunin, serine protease inhibitor domains, and fragments thereof.
Claims9
A substantially purified protein, having serine protease inhibitory activity, selected from the group of proteins consisting of materials each of which comprises one of the following amino acid sequences, the amino acids of said sequences being numbered in accordance with the amino acid sequence of native human placental bikunin shown in FIG. 4F in which the N-terminal residue generated by removal of signal peptide is designated as residue 1:
- AGSFLAWL GSLLLSGVLA −1 ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQQ ENPPLPLGSK VVVLAGAVS 179 (SEQ ID NO:
- 2);
- MLR AEADGVSRLL GSLLLSGVLA −1 ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQQ ENPPLPLGSK VVVLAGLFVM VLILFLGASM VYLIRVARRN 200 QERALRTVWS SGDDKEQLVK NTYVL 225 (SEQ ID NO:
- 45);
- MAQLCGL RRSRAFLALL GSLLLSGVLA −1 ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQQ ENPPLPLGSK VVVLAGLFVM VLILFLGASM VYLIRVARRN 200 QERALRTVWS FGD 213 (SEQ ID NO:
- 47);
- and ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 25 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 75 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 125 ACMLRCFRQQ ENPPLPLGSK VVVLAGAVS 179 (SEQ ID NO.:
- 1);.
A substantially purified serine protease inhibitor protein containing at least one Kunitz-like domain comprising an amino acid sequence:
- ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50
- YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DS 92
- (SEQ ID NO.:
- 8) with the proviso that the protein does not have the amino acid sequence of SEQ ID NOS.:49 and b 71.
A substantially purified protein, having serine protease inhibitory activity, selected from the group of proteins consisting of materials each of which consists one of the following amino acid sequences, the amino acids of said sequences being numbered in accordance with the amino acid sequence of native human placental bikunin shown in FIG. 4F in which the N-terminal residue generated by removal of the signal peptide is designated as residue 1:
- ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSESHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQQ ENPPLPLGSK 170 (SEQ ID NO:52);
- AGSFLAWL GSLLLSGVLA −1 ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQQ ENPPLPLGSK VVVLAGVS 179 (SEQ ID NO:2);
- MLR AEADGVSRLL GSLLLSGVLA −1 ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTFNATG.DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQQ ENPPLPLGSK VVVLAGLFVM VLILFLGASM VYLIRVARRN 200 QERALRTVWS SGDDKEQLVK NTYVL 225 (SEQ ID NO:45);
- MAQLCGL RRSRAFLALL GSLLLSGVLA −1 ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQQ ENPPLPLGSK VVVLAGLFVM VLILFLGASM VYLIRVARRN 200 QERALRTVWS FGD 213 (SEQ ID NO:47);
- ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS QNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQQ ENPPLPLGSK VJVLAGLFVM VLILFLGASM VYLIRVARRN 200 QERALRTVWS FGD 213 (SEQ ID NO:70);
- IHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLEV YGGCDGNSNN 50 YLTKEECLKK CATV 64 (SEQ ID NO:4);
- CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK C 61 (SEQ ID NO.:5);
- YEEYCTANA VTGPCPASFP RWYFDVERNS QNNFIYGGCR GNKNSYRSEE 150 ACMLRC FRQ 159 (SEQ ID NO:6);
- CTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRC 156 (SEQ ID NO:7);
- IHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQ 159 (SEQ ID NO:3);
- CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRC 156 (SEQ ID NO:50);
- ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNSAD SSVPSAPRRQ DSEDHSSDMF 100 NYEEYCTANA YTGPCPASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE 150 ACMLRCFRQQ ENPPLPLGSK VVVLAGAVS 179 (SEQ ID NO:1);
- and ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN 50 YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DS 92 (SEQ ID NO:8).
Dependencies are inferred from the claim text.